Conformational dynamics has an established role in enzyme catalysis, but its contribution to ligand binding and specificity is largely unexplored. Here we used the Tiam1 PDZ domain and an engineered variant (QM PDZ) with broadened specificity to investigate the role of structure and conformational dynamics in molecular recognition. Crystal structures of the QM PDZ domain both free and bound to ...

Because water dissolves in silicate melts as at least two species, H O molecules and OH groups, the exsolution 2 Ž . evaporation enthalpy of the H O component from a silicate melt depends on species proportions, which in turn depends on 2 H O pressure. In this short communication, a formal analysis of the exsolution enthalpy of water from a silicate melt is 2 presented, taking into account of t...

Abstract Recently, one of the authors suggested calculating void fraction, an essential element in thermalhydraulics, working with the ‘thermodynamic’ quality instead of the usual ‘flow’ quality. However, the standard heat balance is currently stated as a function of the ‘flow’ quality. Therefore, we should search a new energy balance between the mixture enthalpy, based on ‘thermodynamic’ quali...

Starch and proteins are major components in the wheat endosperm that affect its end product quality. Between the two textural classes of wheat i.e. hard and soft, starch granules are loosely bound with the lipids and proteins in soft wheat due to higher expression of interfering grain softness proteins. It might have impact on starch granules properties. In this work for the first time the phys...

The ultrasonic absorption, alpha lambda, as a function of temperature and frequency was determined in large unilamellar vesicles (LUVs) in which specific phospholipid side chains were deuterated. Deuteration significantly altered the temperature and frequency dependence of alpha lambda. The frequency change was especially marked, with decreased frequency and broadening of the ultrasound relaxat...

Dielectric relaxation studies of 4-n-octyl-4'-cyanobiphenyl (8 CB) were performed in the pressure range 0 .1 -1 2 0 MPa, the frequency range 1 k H z-1 3 MHz and the temperature range 295-331 K. The dielectric behaviour of 8CB in the nematic phase is similar to that of 5CB, 6CB and 7CB. In particular, the activation enthalpy decreases with increasing pressure and the activation volume decreases ...

In the present work, ternary special quasirandom structures SQSs for a fcc solid solution phase are generated at different compositions, xA=xB=xC= 1 3 and xA= 1 2 , xB=xC= 1 4 , whose correlation functions are satisfactorily close to those of a random fcc solution. The generated SQSs are used to calculate the mixing enthalpy of the fcc phase in the Ca-Sr-Yb system. It is observed that first-pri...

We introduce a Raman profiling method to track homogenization of GexSe100−x melts in real time, and show that 2 g melts reacted at 950 °C in high vacuum homogenize in 168 h on a scale of 10μm. Homogenization of melts is precursive to self-organization of glasses. In the present glasses, compositional variation of Raman active corner-sharingmode frequency of GeSe4 units,molar volumes, and the en...

The effect of thermal cycling treatments on the thermal stability and mechanical properties of a Ti48Zr20Nb12Cu5Be15 bulk metallic glass composite (BMGC) has been investigated. Results show that moderate thermal cycles in a temperature range of −196 ◦C (cryogenic temperature, CT) to 25 ◦C (room temperature, RT) or annealing time at CT has not induced obvious changes of thermal stability and the...

Many RNAs need Mg2+to produce stable tertiary structures. Here we describe a simple method to measure the rate and activation parameters of tertiary structure unfolding that exploits this Mg2+dependence. Our approach is based on mixing an RNA solution with excess EDTA in a stopped-flow instrument equipped with an absorbance detector, under conditions of temperature and ionic strength where, aft...