In this study, Gracilaria verrucosa, red seaweed has been used for production of agar and bioethanol. The algae harvested at various time durations resulted in extraction of ~27-33% agar. The leftover pulp was found to contain ~62-68% holocellulose, which on enzymatic hydrolysis yielded 0.87 g sugars/g cellulose. The enzymatic hydrolysate on fermentation with Saccharomyces cerevisiae produced e...

In this study, we tried explant method and a combination of explant-enzymatic method for the extraction of Mesenchymal Stem Cells from ovine umbilical cord tissue wharton’s jelly. The isolated and culture expanded cells expressed strong alkaline phosphatase activity and they were shown to form colonies. The Mesenchymal Stem Cells showed positive for CD44 and negative for CD34 surface markers by...

The influence of oil rate and quality of rice bran oil by enzymatic extraction ultrasonic assisted were studied through designed experiment. The optimum enzymatic hydrolysis conditions were determined by the way of the orthogonal test. The results showed: after lipase enzyme in the rice bran (40 mesh sieve) was killed by the hot steam, the solution compounding material liquid ratio was 1:5 (W/V...

Wine lees, a sludge-like material mainly consisting of yeast cells, are rich in mannoproteins. However, no reports on the extraction mannoproteins from wine lees to be used as winemaking additives available. This study aimed at developing efficient methods for glycocompounds and assess their impacts when added back wine. White were extracted using physical (autoclave ultrasonication) enzymatic ...

Resveratrol is a natural compound that has many beneficial physiological functions. In recent years, the research on resveratrol gradually become hotspot. This article summarized literature reports all over world, classified and concluded extraction technology, function nano-delivery carriers of resveratrol. Solvent extraction, microwave-and ultrasonic-assisted supercritical carbon dioxide enzy...

Microalgal biodiesel is one of the most promising renewable fuels. The wet technique for lipids extraction has advantages over the dry method, such as energy-saving and shorter procedure. The cell disruption is a key factor in wet oil extraction to facilitate the intracellular oil release. Ultrasonication, high-pressure homogenization, enzymatic hydrolysis and the combination of enzymatic hydro...

The many recent dry-grind plants that convert corn to ethanol are potential sources of substantial amounts of corn oil. This report describes an aqueous enzymatic extraction method to separate oil from dry-fractionated corn germ. The method is an extension of a method previously developed for wet-mill germ. Oil dispersed in lipid bodies throughout the germ was converted to oil droplets suspende...

DNA is often subjected to multiple enzymatic treatments during subcloning procedures. For example, converting a restriction site to another by insertion of a synthetic linker requires five or six enzymatic steps: restriction enzyme digestion, treatment with DNA polymerase (if ends are not blunt), phosphorylation of linkers, ligation of linkers to blunt ends, removal of excess linkers by restric...

The study was carried out to optimize pumpkin oil recovery in the process of aqueous extraction preceded by enzymatic maceration of seeds, as well as to compare the quality of the obtained oil to the quality of cold-pressed pumpkin seed oil. Hydrated pulp of hulless pumpkin seeds was macerated using a 2% (by mass) cocktail of commercial pectinolytic, cellulolytic and proteolytic preparations (R...

Jamun (Syzygium cuminii L.) is one of the important indigenous minor fruit with high medicinal value. The jamun cultivation is unorganized and there is huge loss of this fruit every year. The perishable nature of the fruit makes its postharvest management further difficult. Due to the strong cell wall structure of pectin-protein bonds and hard seeds, extraction of juice becomes difficult. Enzym...