Characterization, understanding and overcoming barriers of enzymatic hydrolysis of different raw materials is essential for the development of economically competitive processes based on enzymatic treatments. This work focused on factors relevant for the improvement of enzymatic hydrolysis of lignocellulose raw materials derived from softwood. The major interest of the work was in lignin. Speci...

The mechanism of the release of polypeptide chains from the ribosome-messenger RNA complex is not fully understood. It has been reported that free polypeptide chains are formed in cell-free protein-synthesizing systems, directed by polyribonucleotides, only if these polynucleotides contain statistically high frequencies of UAA codons.'-5 However, we do not know how the chain is released from th...

Current research and development in cellulosic ethanol production has been focused mainly on agricultural residues and dedicated energy crops such as corn stover and switchgrass; however, woody biomass remains a very important feedstock for ethanol production. The precise composition of hemicellulose in the wood is strongly dependent on the plant species, therefore different types of enzymes ar...

Changes in the foaming properties of whey proteins concentrate (WPC) subjected to enzymatic hydrolysis were studied. Activity and stability of the foam generated from the enzymatic hydrolysis of WPC with two proteases: Trypsin from porcine pancreas (analytical quality) and Alcalase 2.4 L FG (Alcalase) (commercial food grade) were analyzed. WPC solution at concentration 100 mg/mL in 50 mM phosph...

The present study investigates the hydrolysis, microstructural profiling and utilization of guar gum (Cyamopsis tetragonoloba) as a prebiotic in yoghurt. Guar galactomannans (GG) was purified partially depolymerized using an acid, alkali enzyme to improve its characteristics increase utilization. potential hydrolyzed determined Basel supplemented media. Crude (CGG), (PGG), base (BHGG), acid (AH...

Enzymatic hydrolysis of non-dried and dried cellulose samples having various particles size, degree of polymerization, porosity, crystalline polymorph, and content of non-crystalline domains has been studied. Regression analysis was carried out to determine contribution of various structural features of cellulose samples to their hydrolysability. It was found that particle size, degree of polym...

Waste palm dates were subjected to analysis for composition and enzymatic hydrolysis of their flesh fibers. The fruit contained 32% glucose and 30% fructose, while the water-insoluble fibers of its flesh consisted of 49.9% lignin and 20.9% polysaccharides. Water-insoluble fibers were settled to 55% of its initial volume in 12 h. The presence of skin and flesh colloidal fibers results in high vi...

In the course of studies on the desamidation of glutamine in rat liver extracts marked acceleration of this reaction was noted in the presence of 2,4-diketovaleric acid (acetopyruvic acid) .l Recent work indicates that pyruvic acid and certain other ar-keto acids increase the desamidation of glutamine in aqueous extracts of normal and neoplastic rat liver (l-3). Investigation of the mechanism o...

Väljamäe, P. 2002. The kinetics of cellulose enzymatic hydrolysis: implications of the synergism between enzymes. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 781. 54 pp. Uppsala. ISBN 91-554-5479-8. The hydrolysis kinetics of bacterial cellulose and its derivatives by Trichoderma reesei cellulases was studied. The c...

Cereal Chem. 84(1):61–66 Sorghum bran has potential to serve as a low-cost feedstock for production of fuel ethanol. Sorghum bran from a decortication process (10%) was used for this study. The approximate chemical composition of sorghum bran was 30% starch, 18% hemicellulose, 11% cellulose, 11% protein, 10% crude fat, and 3% ash. The objective of this research was to evaluate the effectiveness...