In order to study on different methods of application of polyamines (putrescine, spermine, spermidine) on some morphological, physiological, enzymatic and vase life of Rosa hybrida cv. ‘Dolce Vita’, experiment was conducted as a factorial experiment in a completely randomized design with 32 treatments, 3 replicates and each replication containing 5 vases, a total of 480 vases were executed. Fir...

phenylketonuria (pku) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. the symptoms of this disease can be prevented if detected soon after birth. therefore, blood phenylalanine (phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of pku patients. the goal of this research was to introduce a r...

Metabolic pathway databases such as KEGG contain information on thousands of enzymatic reactions drawn from the biomedical literature. Ensuring consistency of such large metabolic pathways is essential to their proper use. In this paper, we develop an efficient method to determine consistency of an important class of enzymatic reactions, and test the method on the latest release of the KEGG LIG...

We describe an HPLC method for quantifying creatinine, separating the analyte from other compounds in serum and urine by cation-exchange chromatography and measuring its absorbance at 234 nm. The precision of the method (CV) varied from 2.9% (mean creatinine concentration, 31 mumol/L) to 1.7% (361 mumol/L) within a series of assays and from 3.9% (34 mumol/L) to 2.4% (391 mumol/L) between series...

BACKGROUND The Jaffe and enzymatic methods are the two most common methods for measuring serum creatinine. The Jaffe method is less expensive than the enzymatic method but is also more susceptible to interferences. Interferences can lead to misdiagnosis but interferences may vary by patient population. The overall risk associated with the Jaffe method depends on the probability of misclassifica...

L-lactate, a key metabolite of the anaerobic glycolytic pathway, plays an important role as a biomarker in medicine, in the nutritional sector and food quality control. For these reasons, there is a need for very specific, sensitive, and simple analytical methods for the accurate L-lactate measuring. A new highly selective enzymatic method for L-lactate determination based on the use of flavocy...

Given the substantial computational requirements of stochastic simulation, approximation is essential for efficient analysis of any realistic biochemical system. This paper introduces a new approximation method to reduce the computational cost of stochastic simulations of an enzymatic reaction scheme which in biochemical systems often includes rapidly changing fast reactions with enzyme and enz...

The enzymatic method for the determination of serum creatinine is accepted as one of the standard method in a clinical laboratory. The enzymatic method for the determination of serum creatinine was optimized for use with Merilyzer AutoQuant-400 auto analyzer and its performance characteristics were practically compared with Jaffe’s Kinetic. Effects of some interfering substances like Serum bili...

Pectin is a natural polysaccharide used in food and pharma industries. Pectin degree of methylation is an important parameter having significant influence on pectin applications. A rapid, fully automated, kinetic flow method for determination of pectin methyl esters has been developed. The method is based on a lab-made analyzer using the reverse flow-injection/stopped flow principle. Methanol i...

1 (JRING INVESTIGATIONS of the renal reabsorptive mechanism for glucose, a method to quantitate adequately the glucose of normal urine (1-3) was required. The nonspecificity of classic methods for reducing sugars makes them inadequate for application to normal urine. Specific glucose oxidase methods have not yielded adequate recoveries when applied to normal, dilute urine. I have adopted the en...