background : microbial monitoring of aquatic sources is essential in the correct management of these vital sources. multiple tube fermentation (mtf) technique is currently used for assessment of the microbial quality of water sources. however, today enzymatic assay has been presented as an alternative approach for detecting indicator bacteria. the purpose of this study was to compare enzymatic ...

CHROMagar Candida medium was inoculated with a variety of clinical samples (n = 282) by using membrane filtration instead of swabbing or streaking. This modified CHROMagar procedure increased the ability to detect yeast by 16.7%. Compared to an enzymatic two-step membrane filtration method with fluorogenic substrates, it yielded similar recovery but was slower.

L-lactate, a key metabolite of the anaerobic glycolytic pathway, plays an important role as a biomarker in medicine, in the nutritional sector and food quality control. For these reasons, there is a need for very specific, sensitive, and simple analytical methods for the accurate L-lactate measuring. A new highly selective enzymatic method for L-lactate determination based on the use of flavocy...

The purification of the lignin peroxidase from the juice of Musa paradisiaca stem using a simple procedure involving concentration by ultrafitration and anion exchange chromatography on DEAE cellulose column has been reported.The enzymatic properties of the purified enzyme have been found to be similar to the enzymes reported from the fungal sources.

The sensitivity and reproducibility of the radioenzymatic adenylylating assay for spectinomycin were improved by modifications of the assay procedure and by partial purification of the enzyme. The presence of Bacillus subtilis ribosomes or S-150 fraction in the enzymatic reaction mixture interfered with the ability to measure spectinomycin by this method.

HAVE ltEl’ORTED a comparison of methods for determining urniary pregnanediol and have shown that enzymatic hydrolysis of preglianelhiOl conjugate, preliminary purification using silica gel chromatography, anti gas chromatographic quantitation was the prefered method (1). hi this report we show a simple modification of this procedure that enables the simultaneous determination of pregnanediol ($...

The irreversible Michael addition of thiols to acrylamides is reported as a new tool for the kinetic target-guided synthesis. In an unprecedented enzymatic hydrolysis/thio-Michael addition procedure, potent and selective acetylcholinesterase inhibitors are assembled by the enzyme using both its esterasic and templating ability.

Enzymatic synthesis and the reverse transcription of RNAs containing 2'-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2'-O-carbamoyl uridine triphosphate (UcmTP). UcmTP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.

An improved enzymatic method for the synthesis of adenylysulfate (APS) from adenosine 5'-phosphate using APS-reductase from Thiobacillus denitrificans is described. Isolation of millimole quantitities of this sulfur nucleotide is achieved rapidly by means of ion exchange chromatography on a strongly basic ion exchange resin. A facile and reproducible desalting procedure is described.

A micro-enzymatic method was developed to measure total cholesterol (CHOL) and triglyceride (TG) in lipoproteins and their subfractions separated by density gradient ultracentrifugation. This method had a detection limit and sensitivity below 2 mg/dl and accuracy (bias to reference sera) and imprecision (coefficient of variation) of less than 3% between 2 and 30 mg/dl for both CHOL and TG. In a...