Background: Diabetes mellitus (DM) is a complex metabolic disorder which involves multiple pathology manifested with increased blood glucose, neural degeneration, chronic inflammation, organ dysfunction etc. Hyperactive enzymes like alpha amylase and glucosidase that are involved in digestion of starch sucrose further upswings the postprandial hyperglycaemia by rushing excess glucose moieties i...

The dietary triacylglycerol (TAG) gets absorbed and accumulated in the body through monoacylglycerol (MAG) pathway, which plays a major role obesity related disorders. main enzyme of this acyltransferase 2 (MGAT2), is considered as potential target for developing antiobesity compounds. Hence, there need vitro cell-based assays screening leads MGAT2 inhibitors. Because synthetic inhibitor’s side...

To quantify the antiviral effect of interferon (IFN) we applied a mixture of two horseradish peroxidase-labelled monoclonal antibodies, specific for the E1 glycoprotein of Semliki Forest virus, in a direct enzyme immunoassay. This assay is suitable for detection of virus replication in L-cells, seeded as monolayers in 96-well plates. Inhibition of absorbance values caused by IFN was determined ...

In this study, some pyrazolone-based Schiff base derivatives 2a-e (except 2a) were synthesized for the first time and structurally illuminated by spectroscopic techniques (1H, 13C NMR, FT-IR) elemental analysis. All molecules screened their anticancer antioxidant activities, as well AChE, BChE, tyrosinase inhibitory properties. The obtained results exhibited that compounds 1b (IC50 = 9.497 μΜ) ...

To explore the effect and molecular mechanism of total flavonoids Astragalus on apoptosis inflammation alveolar epithelial cells induced by tumor necrosis factor alpha. Human were divided into control group, alpha alpha+trifluoroacetic acid-low, acid-medium, acid-high alpha+anti-microRNA-NC alpha+microRNA-221 inhibitor acid-high+microRNA-NC acid-H+microRNA-221 mimic group. Real-time fluorescenc...

Mannan synthetase activity in spheroplast lysates prepared from Saccharomyces cerevisiae was measured by following the incorporation of [14C]mannose from guanosine 5'-diphosphate-[14C]mannose into material precipitable with cold 0.3 M perchloric acid. When enzyme activity was assayed at high concentrations of spheroplast lysate protein (10 mg/ml) in the presence of 7.5 mM MnCl2, a severe inhibi...