This study aims to determine of Chloramphenicol test for Vannamei shrimp conducted ELISA (Enzyme-Linked Immunosorbent Assay) method with 1 treatment. is done by adding the Abenzyme conjugate, and ends addition substrate reaction stop buffer. Each treatment was performed three times. The value (CAP) (Litopenaeus vannamei) which tested at 4 weeks ranged from 0.021 0.153 ppb. result showed in stil...

there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

diagnosis of toxoplasma gondii (t.gondii) infection is of great medical importance especially for pregnant women and immunosuppressed patients. numerous studies have shown that the recombinant production of several ttoxoplasma antigens, including dense granule antigens (gras) has a great potential as diagnostic reagents. previous studies reported expression of amino terminal gra8 protein in fus...

results almost 8% of the personnel showed positive tst (over 10 mm) reaction while 29% of them had considerable t - cell reactivity against ppd in elispot assays. however, the esat - 6 reactivity was found only in one case of hcws. no correlation was found between the patterns of the reactions and the age or the duration of the employment or previous vaccination history of the participants. the...

an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...