Abstract Asherman syndrome (AS) refers to the loss of endometrial stem cells and matrix hyperplasia caused by basal layer injury. Its common clinical manifestations include narrowing uterine cavity, reduction in menstrual volume, abdominal pain, amenorrhea, infertility. According cause AS, treatment includes a mechanical barrier functional recovery. Because recovery endometrium depends on regen...

In cattle, conceptus-maternal interactions are critical for the establishment and maintenance of pregnancy. A major component of this early interaction involves the transport of nutrients and secretion of key molecules by uterine epithelial cells to help support conceptus development during the peri-implantation period of pregnancy. Objectives were to: 1) analyze temporal changes in the amino a...

Objective: Our aim was to investigate the effects of levonorgestrel-releasing intrauterine system on the endometrium of ovariectomized rats under estrogen replacement therapy. Methods: Twenty-four Sprague-Dawley rats were divided into four groups and operated for the insertion of levonorgestrelreleasing (3 μg/day) or placebo-bearing intrauterine devices (IUD). Following the operation, the rats ...

Luminal epithelial, glandular epithelial, and stromal cells were isolated from pig endometrium by enzymatic dispersion and sieve filtration. The three cell types, maintained in primary culture, showed distinctly different morphologies when viewed by light and scanning electron microscopy. Immunocytochemical staining indicated that luminal and glandular epithelial cells were positive for both cy...

This study was undertaken to determine the modulation of uterine function by chorionic gonadotrophin (CG) in a nonhuman primate. Infusion of recombinant human CG (hCG) between days 6 and 10 post ovulation initiated the endoreplication of the uterine surface epithelium to form distinct epithelial plaques. These plaque cells stained intensely for cytokeratin and the proliferating cell nuclear ant...

Implantation in humans is a complex process that involves embryo apposition and attachment to the maternal endometrial epithelium, traversing adjacent cells of the epithelial lining, and invasion into the endometrial stroma. These processes involve a variety of molecules which are not unique in themselves, but play unique roles in the process of implantation. The molecular dialogue that occurs ...

It has been demonstrated that human endometrial stromal cells (ESC) produce a variety of chemokines in vivo and in vitro. To evaluate the expression of epithelial neutrophil-activating peptide 78 (ENA-78) in the endometrium, concentrations of ENA-78 in cyclic endometrial tissues were measured using enzyme-linked immunosorbent assay. The expression of ENA-78 was also detected in cyclic endometri...

Embryonic implantation is a crucial event for the human reproductive function. Cytokines and paracrine molecules have been proposed as putative local regulators of this process. The leptin or the OB protein has been linked to the reproductive function and inflammatory response. In the present study, we describe for the first time the expression of leptin and leptin receptor (long form) in the s...

The method of Graham and Karnovsky (1) for the fine structural localization of peroxidase has been used for visualization of endogenous peroxidases in a variety of cells (2-5) known to contain peroxidase by biochemical analysis .' . 2 In leukocytes (2), eosinophils (3, 4), 1 and macrophages,' as well as in thyroid and salivary gland epithelial cells (5), 2 Kupffer cells of the liver (5),3 and e...

background: pinopodes are suggested as biological markers of uterine receptivity, but their molecular components are unknown. objective: co-expression of galectin-3 and ?v?3 integrin at human pinopodes has been examined in this study to propose a role for them during adhesion phase of embryo implantation. materials and methods: biopsies were obtained from early and mid luteal phase endometrium ...