background :  nosocomial infections are considered as health issues that affect developed and poor countries. these infections can be transmitted to patients through hospital environment and equipments. the aim of this study was to evaluate the prevalence of gram-negative bacilli in the environment and the equipments of hospitals in the east of golestan province in 2015.   methods :  in this st...

Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in the intensive care unit and is associated with major morbidity and attributable mortality. Strategies to prevent VAP are likely to be successful only if based upon a sound understanding of pathogenesis and epidemiology. The major route for acquiring endemic VAP is oropharyngeal colonization by the endogenous flora ...

Ultrasound biomicroscopy (UBM) is being used increasingly in cataract and refractive surgery to evaluate phacomorphic lenses, dislocated intraocular lenses, misplaced haptics, sulcus-to-sulcus measurements for implantable Collamer lenses (ICLs), ciliary body cysts before ICL implantation, retained lens fragments, and anterior effusions. In a previous report, biometry apparatus grew pathogenic f...

By simulating the operating conditions, it is possible to verify and evaluate technical properties of motor vehicle oil filters functionality designed equipment. Contaminated engine from operation was used with MANN W950/26 filter a CNH Industrial 2992242 in test circuit. Before use, level contamination circuit determined by evaluating physicochemical properties. The laboratory equipment also a...

Polymorphism discovery is a routine application of next-generation sequencing technology where multiple samples are sent to a service provider for library preparation, subsequent sequencing, and bioinformatic analyses. The decreasing cost and advances in multiplexing approaches have made it possible to analyze hundreds of samples at a reasonable cost. However, because of the manual steps involv...

All surgical operations have the potential for contamination, and the equipment used can harbour bacteria. We collected samples from 100 elective primary hip and knee arthroplasties. These showed rates of contamination of 11.4% for the sucker tips, 14.5% for light handles, 9.4% for skin blades and 3.2% for the inside blades used during surgery; 28.7% of gloves used for preparation were also con...

Concern about contamination of fibreoptic endoscopes with human immunodeficiency virus (HIV) has generated a variety of disruptive and possibly unnecessary infection control practices in endoscopy units. Current recommendations on the cleaning and disinfection of endoscopes have been formulated without applied experimental evidence of the effective removal of HIV from endoscopes. To study the k...

This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets in Surabaya and Yogyakarta regions, Indonesia. To confirm the effectiveness and specificity of this fragment, thirty nine DNA samples from different meatball shops were isolated and amplified, and...

Occupational exposure to cytotoxic drugs of hospital personnel involved in their preparation and administration is a major issue: ever since the introduction of protective measures in recent decades, the handling of these drugs has always been referred to as an occupational health hazard. Isolator technology was one of the protective equipments aimed at providing safe handling, but it has not y...