The mechanism of action of efinaconazole, a new triazole antifungal, was investigated with Trichophyton mentagrophytes and Candida albicans. Efinaconazole dose-dependently decreased ergosterol production and accumulated 4,4-dimethylsterols and 4α-methylsterols at concentrations below its MICs. Efinaconazole induced morphological and ultrastructural changes in T. mentagrophytes hyphae that becam...

represent a minor source of cholesterol (Ibanez et al., 2000). In this work we analyzed the sterol content of agricultural soils. Ergosterol is the main endogenous sterol of fungi and Three eukaryotic sterols, cholesterol, -sitosterol, and ergosterol were of some microalgae. Ergosterol content in fungal cells chosen as representative of the animal, plant, and fungal kingdoms, varies in differen...

Ajuga remota is the most frequently used medicinal herb for malaria treatment in Kenya. Its two known isolates ajugarin-1 (1) and ergosterol-5,8-endoperoxide (3) and a new isolate 8-O-acetylharpagide (2) were evaluated for their in vitro antiplasmodial activity. Ajugarin-1 was moderately active, with an IC(50) of 23.0 +/- 3.0 microM, as compared to chloroquine (IC(50) = 0.041 +/- 0.003 microM) ...

This paper deals with the effect of Tween 80 and ergosterol supplementation to the 24oPt wort with 30% maltose syrup adjunct on the fermentation performance of the immobilized yeast in calcium alginate gel. The higher the content of supplements in the wort, the higher the yeast growth, the sugar and free amino nitrogen consumption rates and the ethanol production rate but the lower the ethanol ...

Different fungi are discussed to play a certain role in recent spruce decline in addition to different abiotic factors such as S 0 2, photooxidants (ozone) or unfavourable climatic conditions. Thus, ergosterol as a specific fungal sterol was used as a biochemical indicator for fungal infection in differently damaged spruce and fir needles. With its aid it could be demonstrated that the importan...

There is no effective treatment for the prevalent chronic form of Chagas' disease in Latin America. Its causative agent, the protozoan parasite Trypanosoma cruzi, has an essential requirement for ergosterol, and ergosterol biosynthesis inhibitors, such as the antifungal drug posaconazole, have potent trypanocidal activity. The antiarrhythmic compound amiodarone, frequently prescribed for the sy...

Antifungal activity of Bacillus coagulans creates the possibility to use this microorganism in biological control of fungal plant pathogens. Activity of Bacillus coagulans (No 6), isolated from lupine compost, against seven pathogenic species of indicator fungi: Bipolaris sorokiniana, Trichothecium roseum, Rhizoctonia solani, Sclerotinia sclerotiorum, Fusatium oxysporum, Fusarium solani and Fus...

Antifungal azoles inhibit ergosterol biosynthesis by interfering with lanosterol 14α-demethylase. In this study, seven upregulated and four downregulated ergosterol biosynthesis genes in response to ketoconazole treatment were identified in Neurospora crassa. Azole sensitivity test of knockout mutants for six ketoconazole-upregulated genes in ergosterol biosynthesis revealed that deletion of on...

Cultured mycelium Cordyceps sinensis (CMCS) is a Chinese herbal medicine, which is widely used for a variety of diseases including liver injury in clinic. The current study aims to investigate the protective effects of CMCS against liver fibrosis and to exploit its active antifibrotic substances in vivo and in vitro. For evaluating the antifibrotic effect of CMCS and ergosterol, male C57BL/6 mi...

Ergosterol and 3-hydroxy fatty acids, chemical markers for fungal biomass and the endotoxin of gram-negative bacteria, respectively, may be useful in studies of health effects of organic dusts, including domestic house dust. This paper reports a method for the combined determination of ergosterol and 3-hydroxy fatty acids in a single dust sample and a comparison of these chemical biomarkers det...