Bacterial transporters mediate the exchanges between intracellular and extracellular environments. Modification of transport route could be applied to speed up the metabolic reactions and promote the production of aimed compounds. Herein, lysine 2-monooxygenase (DavB) and δ-aminovaleramidase (DavA) were co-expressed in Escherichia coli BL21(DE3) to produce nylon-5 monomer 5-aminovalerate from l...

Fundamental aspects of outer membrane vesicle (OMV) biogenesis and the engineering producer strains have been major research foci for many in recent years. The focus this study was OMV production a variety Escherichia coli including wild type (WT) (K12 BW25113), mutants (from Keio collection) proprietary [BL21 BL21 (DE3)] strains. present investigated proteome prospective mechanism that underpi...

Đặt vấn đề: Acid kojic là một tác nhân làm trắng da được sử dụng phổ biến trong mỹ phẩm. sản xuất bằng phương pháp lên men từ nấm, quá trình này tốn nhiều thời gian và giá phẩm tạo ra khá cao. Nghiên cứu gần đây trên Aspergillus oryzae đã chỉ mã hoá gen kojA có liên quan đến phản ứng tổng hợp acid glucose. Mục đích: protein KojA tái tổ dạng dung với SUMO Escherichia coli. Phương pháp: Tạo dòng ...

BACKGROUND In the biopharmaceutical industry, Escherichia coli (E. coli) strains are among the most frequently used bacterial hosts for producing recombinant proteins because they allow a simple process set-up and they are Food and Drug Administration (FDA)-approved for human applications. Widespread use of E. coli in biotechnology has led to the development of many different strains, and selec...

The temperature-sensitive hemagglutinin (Tsh) expressed by strains of avian pathogenic Escherichia (E.) coli (APEC) has both agglutinin and protease activities. Tsh is synthesized as a 140 kDa precursor protein, whose processing results in a 106 kDa passenger domain (Tsh(s)) and a 33 kDa β-domain (Tsh(β)). In this study, both recombinant Tsh (rTsh) and supernatants from APEC, which contain Tsh(...

A recombinant plasmid for expression of full-length human DNA ligase I (phLig-I) was constructed in a plasmid/phage chimeric vector, pTD-T7N, which was derived from pUC118 by oligonucleotide-directed mutagenesis. The insert contained a 2757-base pair coding sequence for a whole human DNA ligase I and an extra ACC codon adjacent to the ATG initiation codon. This ACC codon was required for achiev...

BACKGROUND Filamentous hemagglutinin (FHA) is one of the most important immunoprotective antigens of Bordetella pertussis (B. pertussis) and a major component of the acellular pertussis vaccine. In the present study, three overlapping recombinant fragments from the immunodominant region of FHA were produced and their immunogenicity was investigated. METHODS Three overlapping coding sequences ...

DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2-CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-1 Blue, SCS110, JM109, TOP10 and BL21-(DE3)-PLysS. Hanahan's method was found to be most effective for...