Bisulfite catalyzes transamination of cytidine at the N4 position; the suitability of this reaction for attaching reporter groups to selected cytidine residues in RNA molecules has been investigated. Poly(C) is nearly quantitatively converted to the poly (N4 aminoethyl-C) derivative after 3 hrs at 42 degrees C with ethylene diamine (pK1 = 7.6) and bisulfite. This derivative reacts quantitativel...

Total antioxidant capacity (TAC) of some Rosmarinus officinalis L. (rosemary) extracts was investigated by an “in batch” analytical method based on chemiluminescence (CL). This method is based on a CL reaction between luminol and hydroxyl radicals generated by the hydrogen peroxide in the presence of Co(II) ions complexed with ethylene diamine tetraacetic acid (EDTA) disodium salt, according to...

The universal acceptance of the animal growth-promoting properties of some antibiotics and their wide usage in formulated commercial feeds requires that simple, rapid and reliable analytical methods be available for control purposes. Of the several antibiotics used in poultry rations, penicillin exerts its growth-promoting properties at levels considerably lower than other antibiotics (2 grams ...

In this study, a novel conjugate consisting of glycol chitosan (GCS) and ethylene diamine tetraacetic acid (EDTA) was synthesized and characterized in terms of conjugation and heavy metal ion chelating capacity. Moreover, its potential application as a metalloenzyme inhibitor was evaluated with three thymopoietin oligopeptides in the presence of leucine aminopeptidase. The results from FTIR and...

The aetiology of neurodegenerative diseases (ND) seems to involve susceptibility genes and environmental factors. Toxic metals are considered major environmental pollutants. Following our study of a case of multiple sclerosis (MS) improvement due to removal of aluminium (Al) and other toxic metals, we have examined the possible relationship between Al intoxication and ND. We used the slow intra...

The present study deals with the pre-treatment of sugar cane molasses for the enhanced production of citric acid by Aspergillus niger NG-4. Different metal complexing agents such as potassium ferrocyanide [K4Fe(CN)6] and ethylene diamine tetra acetic acid (EDTA) were also employed for molasses pre-treatment and the concentrations varied from 50-350 ppm. The maximum production of citric acid (36...

A procedure for the determination of liver microsomal glucose-6-phosphatase is described. Homogenization and ultracentrifrigation were used to prepare a precipitate whose character was defined by monitoring the desire enzyme activity which serves as a marker. Activity of the enzyme was determined by means of a sensitive colorimetric reaction for the product, inorganic phosphate. Non-enzymatic h...

Degeneration of elastic lamina and vascular calcification are common features of vascular pathology such as aortic aneurysms. We tested whether dual therapy with targeted nanoparticles (NPs) can remove mineral deposits (by delivery of a chelating agent, ethylene diamine tetraacetic acid (EDTA)) and restore elastic lamina (by delivery of a polyphenol, pentagalloyl glucose (PGG)) to reverse moder...

To understand how Corynebacterium diphtheriae responds to iron limitation, we compared the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein profiles of both wild-type cells and iron uptake mutants grown in either high- or low-iron medium. The removal of iron by ethylene diamine di-(o-hydroxy-phenyl acetic acid) from the growth medium of wild-type cells resulted in in...

Tetra acetyl ethylene diamine (TAED) was used as an activator in H2O2 bleaching to improve bleaching efficiency. The present work was aimed at confirming different activations for various H2O2/TAED bleaching processes, including the addition of acetic anhydride and the stepaddition of sodium hydroxide. The results showed that an acetic anhydride dosage of 1%, an acetic anhydride treatment time ...