Correlative light and electron microscopy (CLEM) is a useful tool for investigating cellular structures at high-resolution. We have extended this technology and developed a new method in which CLEM is combined with live-cell fluorescence imaging, “Live CLEM”. With this method, living cells are first examined by fluorescence microscopy then fixed in situ, and finally the same cells are subjected...

Localization microscopy techniques are super-resolution fluorescence imaging methods based on the detection of individual molecules. Despite the relative simplicity of the microscope setups and the availability of commercial instruments, localization microscopy faces unique challenges. While achieving super-resolution is now routine, issues concerning data analysis and interpretation mean that ...