نتایج جستجو برای: extender

تعداد نتایج: 1636  

Fatemeh Soltanpour Gholamali Moghaddam, Reza Asadpour Sayyed Abbas Rafat

The aim of this study was to evaluate the effects of the addition of antioxidants (vitamin E, β- mercaptoethanol and their combination) to semen extender.  Progressive motility, viability and pH of fresh ram semen were examined. Ejaculate samples were collected with artificial vagina from three Ghezel-Merinose rams (3 years old) during non- breeding reproduction season. Semen was diluted with a...

Hossein Tayefi-Nasrabadi Razi Jafari, Reza Asadpour,

The objective of this study was to evaluate effect of different concentrations of catalase in two extenders on motility, viability and lipid peroxidation bull spermatozoa during semen freezing process. Thirty ejaculates collected from ten Holstein bulls were pooled and evaluated at 37 °C. Pool ejaculated was split into two main experimental groups, 1 and 2. In experiment 1, specimen was dilute...

2006
Jian-hong Hu Qing-Wang Li Xiao-Yu Chen Shu-Shan Zhang Li-Qiang Wang

In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of yolk is largely presumed to be due to low density lipoproteins (LDL). The effects of LDL on sperm quality of bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has been m...

2011
Roland Kozdrowski Wojciech Niżański Andrzej Dubiel Wanda Olech

BACKGROUND European bison is the largest mammal in Europe with the population of approximately 4000 individuals. However, there is no report of post-mortem spermatozoa collection and cryopreservation from this species and the aim of this study was to test if the epididymal spermatozoa collected post-mortem from European bison are suitable for cryopreservation and artificial insemination (AI). ...

2014
Hyunjun Park Brian M. Kevany David H. Dyer Michael G. Thomas Katrina T. Forest Avadhesha Surolia

We have previously shown that the acyl transferase domain of ZmaA (ZmaA-AT) is involved in the biosynthesis of the aminopolyol polyketide/nonribosomal peptide hybrid molecule zwittermicin A from cereus UW85, and that it specifically recognizes the precursor hydroxymalonyl-acyl carrier protein (ACP) and transfers the hydroxymalonyl extender unit to a downstream second ACP via a transacylated AT ...

2015
Zhendong Zhu Xiaoteng Fan Yinghua Lv Nan Zhang Chuning Fan Pengfei Zhang Wenxian Zeng Paul McNeil

The process of cryopreservation results in high concentration of reactive oxygen species which is detrimental to spermatozoa. The aim of this study was to investigate whether addition of vitamin E analogue to freezing extender can facilitate the cryosurvival of spermatozoa in rabbits, and how vitamin E protects spermatozoa against damages during the process of preservation. Freshly ejaculated s...

Journal: :Asian journal of andrology 2005
Ya-Hui Li Ke-Jun Cai Lei Su Mo Guan Xie-Chao He Hong Wang Andras Kovacs Wei-Zhi Ji

AIM To establish a method for cynomolgus monkey sperm cryopreservation in a chemically defined extender. METHODS Semen samples were collected by electro-ejaculation from four sexually mature male cynomolgus monkeys. The spermatozoa were frozen in straws by liquid nitrogen vapor using egg-yolk-free Tes-Tris mTTE synthetic extender and glycerol as cryoprotectant. The effects of glycerol concent...

2016
Lauren Ray Timothy R. Valentic Takeshi Miyazawa David M. Withall Lijiang Song Jacob C. Milligan Hiroyuki Osada Shunji Takahashi Shiou-Chuan Tsai Gregory L. Challis

Type I modular polyketide synthases assemble diverse bioactive natural products. Such multienzymes typically use malonyl and methylmalonyl-CoA building blocks for polyketide chain assembly. However, in several cases more exotic alkylmalonyl-CoA extender units are also known to be incorporated. In all examples studied to date, such unusual extender units are biosynthesized via reductive carboxyl...

2005
Yusuf Bozkurt Ergun Akcay Necmettin Tekin Selcuk Secer

Rainbow trout (Oncorhynchus mykiss) sperm was frozen in liquid nitrogen in straws or as pellets on dry ice and stored in liquid nitrogen until artificial insemination. Sperm was diluted with one of three extenders containing 15% DMSO or 15% DMA as a cryoprotectant at a ratio of one part sperm to two parts extender. The straws were thawed in a water bath while the frozen pellets were thawed in t...

Journal: :Cryo letters 2013
M V Santos M Sansinena N Zaritzky J Chirife

The subject of present work was to predict internal temperature gradients developed during freezing of bovine sperm diluted in extender, packaged in 0.5 ml French plastic straws and suspended in static liquid nitrogen vapor at -100 degree C. For this purpose, a mathematical heat transfer model previously developed to predict freezing times (phase change was considered) of semen/extender package...

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