UNLABELLED Annexin A5 is a phospholipid binding protein with high affinity for phosphatidyl-serine, which is externalized by cells undergoing programmed cell death. An increased programmed cell death rate has been reported in the heart after myocardial infarction (MI). The aim of this study was to correctly localize annexin A5 uptake in vivo and to determine the area at risk in humans with acut...

The enzymatic activities for the synthesis of phosphatidylcholine from phosphatidylethanolamine and Sadenosyl-L-methionine have been solubilized from rat liver microsomes by ultrasonic disruption in the presence of 0.2% Triton X-100. Significant co-purification of the activities was achieved by chromatography of the Triton extract on octyl-Sepharose CL-4B and, subsequently, on Sepharose 6B. Thr...

Antisera to the phosphatides phosphatidyl inositol and phosphatidyl choline as well as to the xanthophylls violaxanthin and zeaxanthin were obtained by immunization of rabbits. The phosphatidyl choline antiserum did not give cross reactions with thylakoid membrane glycolipids, nor with phosphatidyl glycerol and phosphatidyl inositol. The antiserum to phosphatidyl inositol also did not react wit...

The enzymatic activities for the synthesis of phosphatidylcholine from phosphatidylethanolamine and Sadenosyl-L-methionine have been solubilized from rat liver microsomes by ultrasonic disruption in the presence of 0.2% Triton X-100. Significant co-purification of the activities was achieved by chromatography of the Triton extract on octyl-Sepharose CL-4B and, subsequently, on Sepharose 6B. Thr...

When (32)P(1) was supplied as a 15-minute pulse to normal Spirodela oligorrhiza plants, the first phospholipid to become fully labeled was phosphatidic acid. Phosphatidyl glycerol reached maximum labeling before the other major phospholipids. In phosphorus-deficient plants, however, phosphatidyl glycerol became labeled much more slowly than either phosphatidyl choline or phosphatidyl ethanolami...

A new method has been developed which permits the rapid screening of E. coli colonies for mutants with defective enzymes of phospholipid metabolism. In this procedure, a disc of filter paper is pressed down on an agar plate containing several hundred colonies of mutagen-treated cells, after which the paper is lifted off. In the process the colonies are transferred to the paper, giving rise to a...

Abstract The article is concerned with a central contribution of designing to information visualization in the digital humanities. activity characterized as one externalization, instantiation visible or tangible form ideas. A spectrum different interpretations this process existing literature discussed. arguments are illustrated recent practical examples from authors’ own work range cultural or...

Previously we demonstrated high concentrations of desmosterol and docosahexaenoic acid (DHA, 22:6 n-3) in monkey testes and sperm. Desmosterol, a cholesterol precursor, is not present elsewhere in the body. High concentrations of DHA are found elsewhere only in the retina and brain. To examine the distribution of these compounds in the heads and tails of sperm, we separated them and determined ...