background: matrigel (extracellular matrix) can improve the growth of many cell types in vitro. objective: the aim of the present study was to determine the effect of matrigel on the development of 2-4 cells human embryos in culture. material and methods: surplus 2-4 cells human embryos, resulting from icsi, were divided into two groups (control and test). quality of embryos in both groups was ...

GGDEF domain-containing proteins have been implicated in bacterial signal transduction and synthesis of the second messenger molecule cyclic-di-GMP. A number of GGDEF proteins are involved in controlling the formation of extracellular matrices. AdrA (Salmonella enterica serovar Typhimurium) and HmsT (Yersinia pestis) contain GGDEF domains and are required for extracellular cellulose production ...

human diploid fibroblast cells produce a spectrum of necessary growth factors and extracellular matrix (ecm) components essential for growth and proliferation of a variety of other cell types. in this study, the effect of five human embryonic fibroblast cell lines, isolated from liver, lung, skin and foreskin tissues, was investigated. a coculture system analyse was employed to cloning efficien...

To determine the effects of an intact extracellular matrix on collagen synthesis, arterial smooth muscle cells (SMCs) were plated sparsely on a cell-free, SMC-derived matrix and examined the following day. Collagen synthesis during a 5-hour incubation by cells on the matrix was reduced to 67% of the control values obtained from cultures on plastic. Total protein synthesis was unaffected. Treatm...

The vascular trophic effects of angiotensin II (Ang II) in small doses may precede its hypertension-producing effect, and de novo synthesis of components of extracellular matrix may be a requirement for Ang II-stimulated growth. In the present study, therefore, the incorporation of 35SO4 into glycosaminoglycans (synthesis) of aorta and bladder wall of young adult, male Sprague-Dawley rats was m...

In probing how protein synthesis in neurons is coupled to extracellular stimuli, Tcherkezian et al. (2010) discover that the receptor protein DCC anchors components of the translation machinery at the plasma membrane. Binding of DCC to its ligand, netrin, triggers the release of these components to initiate spatially restricted protein synthesis.

Adenosine inhibits rat vascular smooth muscle cell (SMC) growth. However, the effects of adenosine on human vascular SMC proliferation and synthesis of extracellular matrix proteins, such as collagen, are unknown. The objective of this study was to characterize the effects of exogenous and endogenous (SMC-derived) adenosine on human aortic SMC proliferation and collagen synthesis. Growth-arrest...

Green synthesis of metal nanoparticles through biological resources has attracted attention in recent years. The main reason for that, a lot toxic chemicals as reducing and stabilizing agents are used physical chemical methods which known conventional methods. Organisms such plants, fungi, bacteria, algae alternative sources green nanoparticle because their more eco-friendly nature not be threa...

Adenosme mhlblts rat vascular smooth muscle cell (SMC) growth However, the effects of adenosme on human vascular SMC prohferahon and synthesis of extracellular matnx protems, such as collagen, are unknown The objective of this study was to characterize the effects of exogenous and endogenous (SMC-denved) adenosme on human aotic SMC prohferatlon and collagen synthesis Growth-arrested SMCs were s...

Adenosme mhlblts rat vascular smooth muscle cell (SMC) growth However, the effects of adenosme on human vascular SMC prohferahon and synthesis of extracellular matnx protems, such as collagen, are unknown The objective of this study was to characterize the effects of exogenous and endogenous (SMC-denved) adenosme on human aotic SMC prohferatlon and collagen synthesis Growth-arrested SMCs were s...