PURPOSE To separate and quantify four major saponins in the extracts of the skin and the endosperm of seeds of horse chestnut (Aesculus hippocastanum L.) using ultrasonic solvent extraction followed by a high performance liquid chromatography-diode array detector (HPLC-DAD) with positive confirmation by thin layer chromatography (TLC). METHODS The saponins: escin Ia, escin Ib, isoescin Ia and...

In recent years there has been considerable interest in the synthesis and separation of enantiomers of organic compounds especially because of their importance in the biochemistry and pharmaceutical industry. High-performance liquid Chromatography is a very useful method for the direct separation of enantiomers. However, about 30−40 years ago, commercially available chiral stationary phases wer...

After completing the thio-substitution with Lawesson’s reagent, ethanol was found to be effective in decomposition of inherent stoichiometric six-membered-ring byproduct from reagent a highly polarized diethyl thiophosphonate. The treatment significantly simplified following chromatography purification desired thioamide small scale preparation. As scaling up preparation two pincer-type thioamid...

As the serum peptidome gets increasing attention for biomarker discovery, one of the important issues is how to efficiently extract the peptides from highly complex human serum for peptidome analysis. Here we developed a fully automated platform for direct injection, on-line extraction, multidimensional separation and MS detection of peptides present in human serum. A capillary SPE column packe...

Aminoglycosides are antimicrobial agents used frequently in treatment of human and animal diseases caused by aerobic, gram-negative bacteria. Because of the toxicity of these compounds, considerable effort has been attributed to analysis of aminoglycoside content in drug preparations, in serum and urine specimen in therapeutic drug monitoring, and in edible animal tissues in residue control. Th...

background & aim: according to the increase in diseases such as diabetes and obesity, using of saccharose alternatives for producing of low-calorie and sugar-free alternatives, are taken into consideration of researchers and industrialists. among them, steviosidewith the 300 times more sweetness than saccharose has special place, due to is a natural. the aim of this study is optimizing of stevi...

Bauhunia semibifida is among the several plants that have been studied for their medicinal properties. native to India. However, some species found in Africa. It has important uses, such as anti-bacterial, anti-inflammatory and anti-cancerous. This study aimed primarily at carrying out a preliminary phytochemical screening ascertain classes of bioactive compounds present solvent system(s) separ...

many modern analytical methods deal with the trace-level determination of compounds of interest in highly complex environmental samples by means of chromatographic techniques. the introduction of a “clean” sample into an analytical instrument can make analyses easier and prolongs the equipment life. the use of solid-phase extraction (spe) has grown and is a fertile technique of sample preparati...

in this project, eggshell membrane was used as a solid phase extraction sorbent for extraction of morphine followed by hplc analysis. at first, raw eggshell membrane was used and then, because of low adsorption of morphine on untreated eggshell membrane (

Reported herein is the first enantioselective method for simultaneous separation of chiral pharmaceuticals and pesticides using ultra-high-performance liquid chromatography-tandem mass spectrometry. Separation was achieved a ChiralPak IG-U® column (amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase) ethanol as ‘green’ mobile phase organic modifier. Minimum enantiomer resolutions ra...