The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen (Fg-ESA) on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 F. gigantica flukes in 100 ml phosphate buffer saline (PBS) for 2 h at 37 degrees C, and centrifuging the supernatant at 12,000 g at 4 degrees C for 30 min. The protein content of Fg-ESA was adjust...

Nuclear gene markers, phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold), have been developed for precise discrimination of Fasciola flukes instead of internal transcribed spacer 1. In this study, these two genes of 730 Fasciola flukes from eight Asian countries were analyzed. The results were compared with their mitochondrial NADH dehydrogenase subunit 1 (nad1) lineages ...

Most phylogenetic studies using current methods have focused on primary DNA sequence information. However, RNA secondary structures are particularly useful in systematics because they include characteristics that give "morphological" information which is not found in the primary sequence. Also DNA sequence motifs from the internal transcribed spacer (ITS) of the nuclear rRNA repeat are useful f...

The efficacy of essential oils of Cymbopogan nardus (citronella) and Azadirachta indica (neem) on Fasciola gigantica was assessed in vitro. The essential oil of citronella showed a potential anthelmintic activity whereas neem oil did not show any significant effect. Study revealed that 1% citronella oil showed anthelmintic effect on live Fasciola gigantica. The in vitro anthelmintic effect was ...

abstract background: we aimed to describe morphological and morphometrical characteristics of fasciola spp. in livestock from ardabil province, northwest iran. methods: forty adult flukes were collected from different definitive hosts (cattle and sheep). previously specimens were identified as f. hepatica or f. gigantica based on pcr-rflp of the its-1 region with rsai enzyme.  we identified fas...

Dynamics of serum liver enzymes in rabbits experimentally infected with metacercariae of Fasciola sp. (intermediate form between Fasciola hepatica and F. gigantica) were monitored. Gradual increase of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were observed from 3 weeks post-inoculation (WPI) and peaked at 6 WPI, which corresponded well to the period of migration ...

Serine proteases form one of the most important families of enzymes and perform significant functions in a broad range of biological processes, such as intra- and extracellular protein metabolism, digestion, blood coagulation, regulation of development, and fertilization. A number of serine proteases have been identified in parasitic helminths that have putative roles in parasite development an...

Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase T...