fusarium head blight (fhb) or wheat scab is one of the destructive diseases that cause considerable loss in grain quality in cereal cultivated around the worldwide due to the production of trichotecen mycotoxin in the panicle. type b trichothecenes including nivalenol (niv), deoxynivalenol (don), 3-acetyl deoxynivalenol (3-acdon) and 15-deoxynivalenol (15-acdon) are considered to be major toxin...

Adlay seed samples were collected from three adlay growing regions (Yeoncheon, Hwasun, and Eumseong region) in Korea during 2012. Among all the samples collected, 400 seeds were tested for fungal occurrence by standard blotter and test tube agar methods and different taxonomic groups of fungal genera were detected. The most predominant fungal genera encountered were Fusarium, Phoma, Alternaria,...

A genomic DNA fragment containing Tri6, a transcription activator gene of trichothecene biosynthesis, was cloned by vectorette PCR from Fusarium graminearum F15, which produces type B trichothecene, deoxynivalenol. The nucleotide sequence of the gene showed 84% of identity to that of the type A trichothecene producer Fusarium sporotrichioides NRRL 3299, but the sequence around the initiation co...

The contribution of cell surface proteins to plant pathogenicity of fungi is not well understood. As such, the objective of this study was to investigate the functions and importance of glycosylphosphatidylinositol-anchored proteins (GPI-APs) in the wheat pathogen F. graminearum. GPI-APs are surface proteins that are attached to either the membrane or cell wall. In order to simultaneously disru...

ABSTRACT Fusarium spp. in maize can contaminate the grain with mycotoxins if environmental conditions are favorable for fungal growth. To quantify the relationship between growth of Fusarium spp. and environmental conditions, a mathematical model was developed to simulate growth of F. graminearum and F. verticillioides on maize ears following silk inoculation in field experiments from 1992 to 1...

Removal of introns from transcribed RNA represents a crucial step during the production of mRNA in eukaryotes. Available whole-genome sequences and expressed sequence tags (ESTs) have increased our knowledge of this process and revealed various commonalities among eukaryotes. However, certain aspects of intron structure and diversity are taxon-specific, which can complicate the accuracy of in s...

Total RNA from wheat spikes infected with F. graminearum Fg2 was extracted and the mRNA was purified. Switching Mechanism at 5' end of the RNA Transcript (SMART) technique and CDS Ill/3' primer were used for first-strand cDNA synthesis using reverse transcriptase by RT-PCR. Primer extension polymerase chain reaction was used to construct the double-strand cDNA that was digested by proteinase K,...

To expand our knowledge of Fusarium head blight (FHB) pathogen and trichothecene toxin diversity, a global collection of 2100 isolates was screened for novel genetic variation, resulting in the identification of 16 phylogenetically divergent FHB isolates. The affinities and taxonomic status of these novel isolates were evaluated via phylogenetic analyses of multilocus DNA sequence data (13 gene...

The objective of this study was to determine the frequencies of distribution and the toxigenic potential of Fusarium species isolated from non-harvested maize left in field over winter in 1999 (N = 56) and 2003 (N = 56) in northern Croatia. Zearalenone and trichothecenes (DON, DAS, T-2) were isolated and detected using multitoxin extraction and TLC method. Modified TLC method was applied to det...