Here, we demonstrate detection by mass spectrometry of an intact protein–drug complex directly from liver tissue rats that had been orally dosed with the drug. The comprised fatty acid binding protein 1, FABP1, non-covalently bound to small molecule therapeutic bezafibrate. Moreover, spatial mapping [FABP1+bezafibrate] across a thin section targeted imaging. This work is first demonstration in ...

Obesity, inflammation, and insulin resistance are closely linked. Substance P (SP), via its neurokinin 1 receptor (NK1R), mediates inflammatory and, possibly, neuroendocrine processes. We examined SP effects on lipid storage and cytokine production in 3T3-L1 adipocytes and adipose tissues. 3T3-L1 adipocytes and preadipocytes express NK1R, and 8 days of SP supplementation during differentiation ...

two omega-3 fatty acids including docosahexaenoic acid (dha) and eicosapentaenoic acid (epa) are essential for the physiologic function of neuronal cell membrane. normal function of neuronal cell membrane requires appropriate composition of fatty in its structure. present study was designed to compare the effect of short-term and long-term pretreatment with omega-3 fatty acids on scopolamine-in...

Obesity-induced inflammation is crucial in the pathogenesis of insulin resistance and type 2 diabetes. In this study, we investigated effects Gracilaria chorda (GC) on lipid accumulation obesity-induced inflammatory changes or glucose homeostasis cell models (3T3-L1 adipocytes RAW 264.7 macrophages). Samples GC were extracted using solvents (water, methanol, ethanol) subcritical water (SW) at d...