Cyclin D1 has become a potential target for anti-tumor therapy. Recently, a novel human anti‑cyclin D1 single‑chain variable fragment (AD5) was identified, which demonstrated specific binding activity to cyclin D1 and exhibited anti‑tumor effects. However, the detailed characteristics of AD5 remain unclear. In the present study, the structure and activity of AD5 in the presence of copper II (Cu...

Derivatives of 3,4-hydroxypyridinones have been extensively studied for in vivo Fe3+ sequestration. Deferiprone, a 1,2-dimethyl-3,4-hydroxypyridinone, is now routinely used for clinical treatment of iron overload disease. Hexadentate tris(3,4-hydroxypyridinone) ligands (THP) complex Fe3+ at very low iron concentrations, and their high affinities for oxophilic trivalent metal ions have led to th...

An amperometric sensor was constructed, by using humic acids to immobilize Fe3+ ions on a carbon paste electrode (CPE-HA-Fe), and used for ascorbic acid (H2A) determination. The cyclic voltammogram of the electrode showed electrochemical response due to the Fe3+/Fe2+ couple at E1/2=+0.78 V vs SCE, using 0.5 mol L-1 KCl and 0.2 mol L-1 acetate/0.020 mol L-1 phosphate buffer, at pH = 5.4, as supp...

Bleomycin (BLM) is an anticancer antibiotic isolated from the supernatant of Streptomyces verticillus culture [21]. The cytotoxic activity of BLM depends on generation of singleand double-strand breaks in DNA (for review, see [16]). Indispensable for this BLM activity is the presence of metal ions, specifically Fe2+. Fe2+ BLM complexed with O2 or Fe 3+ BLM complexed with H2O2 leads to formation...

Excesses and shortages of some metal ions such as Fe3+ Cr2O72− pose a threat to human life. A Zn-based two-dimensional MOF has proven be sensitive for in water with Ksv values 1.8 × 104 6.5 104, respectively. The limit detection (LOD) these was found 2.5 μM 0.15 ions, comparative study conducted using two 3D MOFs terms their ability, synthesis structure.

A novel light-emitting monomer M1 and its side-chain polymer P1 containing three conjugated aromatic pendants, including one pyridyl terminus, were successfully synthesized via Wittig and Pd-catalyzed Heck coupling reactions. The fluorescence of polymer P1 was efficiently quenched upon addition of different metal ions due to the facile energy transfers from the pendent groups of polymer P1 to s...

The uptake and subsequent release of iron by apoferritin and ferritin was studied by using labelled iron (59Fe). The experimental results are consistent with predictions arising from amodel system developed in the interpretation ofprevious experiments. In thismodel, uptake and release of ferritin iron is controlled by the available surface area of the small crystalline particles of hydrous ferr...

Human lactoferrins isolated from neutrophilic leucocytes and milk by CM-Sephadex chromatography were similar in Mr (76000) and pI (8.7). Upon acidification, both proteins released their two Fe3+ ions/molecule in a similar biphasic way. Both proteins intravenously injected into mice were cleared from plasma at the same rate. The maximal uptakes by the liver, which occurred 5 min after injection,...

Mesoporous silica functionalized with a 2-hydroxy-naphthyl moiety has been synthesized and characterized by standard techniques like powder X-ray diffraction, N2 adsorption/desorption studies, transmission electron microscopy and spectral studies like FT-IR, UV-visible, fluorescence and 13C and 29Si solid state NMR. The functionalized silica material showed significant enhancement in its emissi...

The weak mechanical properties of hydrogels due to the inefficient dissipation energy in intrinsic structures limit their practical applications. Here, a double-network (DN) hydrogel has been developed by integrating an ionically cross-linked agar network, covalently acrylic acid (AAC) and dynamic reversible coordination between AAC chains Fe3+ ions. proposed model reveals mechanisms improved p...