Optimization of the fed-batch fermentation of Saccharomyces cerevisiae is analyzed. Due to the limited oxygen uptake capacity of the cells, the over ow metabolite ethanol is formed when the substrate concentration is above some critical value. This value decreases during the course of an experiment due to the reduction in dissolved oxygen concentration resulting from biomass formation. Optimal ...

Most large-scale production processes in biotechnology are performed in fed-batch operational mode. In contrast, the screenings for microbial production strains are run in batch mode, which results in the microorganisms being subjected to different physiological conditions. This significantly affects strain selection. To demonstrate differences in ranking during strain selection depending on th...

A dynamic flux balance model based on a genome-scale metabolic network reconstruction is developed for in silico analysis of Saccharomyces cerevisiae metabolism and ethanol production in fed-batch culture. Metabolic engineering strategies previously identified for their enhanced steady-state biomass and/or ethanol yields are evaluated for fed-batch performance in glucose and glucose/xylose medi...

In this paper we study a self-adaptive predictive functional control algorithm as an approach to the control of the temperature in an exothermic atch reactor. The batch reactor is located in a pharmaceutical company in Slovenia and is used in the production of medicines. Due to mixed discrete nd continuous inputs the reactor is considered as a hybrid system. The model of the reactor used for th...

.................................................................................................................................................... 1 I – INTRODUCTION .................................................................................................................................... 2 II – BACKGROUND .................................................................................

The objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant E. coli. Batch and fed-batch culture techniques were employed to grow Escherichia coli BL21 for production of human gamma-interferon in pET expression system. Final cell densities in batch and fed-batch...

This paper provides new insights on the application of the ozonation process for the reduction of the activated sludge production in a sequencing batch reactor (SBR). The study was performed in two identical lab-scale SBRs plant, one for experimental activities (Exp SBR) and one used as control (Control SBR), both fed with domestic sewage. A fraction of the activated sludge collected from the E...

Galvanized steel scrap flow and injection into the HIsarna reactor are investigated using discrete element method (DEM). The particle is fed through an inclined chute hits slag surface where zinc content evaporated solid particles melt. A DEM model setup validated experimental data obtained from exact plant‐scale geometry particles. Using model, effect of inclination, elevation, mode (batch con...

BACKGROUND In this research, the feasibility of, and population dynamics in, one-step anaerobic sequencing batch reactor systems treating the fine sieved fraction (FSF) from raw municipal wastewater was studied under thermophilic (55 °C) and mesophilic (35 °C) conditions. FSF was sequestered from raw municipal wastewater, in the Netherlands, using a rotating belt filter (mesh size 350 micron). ...

The objective of this study was to investigate hydrogen production from alcohol wastewater by anaerobic sequencing batch reactor (ASBR) under thermophillic operation. The ASBR unit used in this study had a liquid holding volume of 4 L and was operated at 6 cycles per day. The seed sludge taken from an upflow anaerobic sludge blanket unit treating the same wastewater was boiled at 95 °C for 15 m...