Recent advances in cytochemistry have revealed that two types of nucleic acids play important roles in the cell (for references, 1 and 2). Whereas these two types of nucleic acids, deoxypentose nucleic acid (DNA) and pentose nucleic acid (PNA), have similar ultraviolet absorption properties, they are distinguished from each other by certain color reactions based on their constituent sugars. Amo...

M~)flkey kidney stable ceIIs developed characterist:c changes when infected with ail known types of African horse-sickness virus. First, there was an increase in the size of ceII nuclei. Then, basophilic Feulgen-positive granules were seen in the v:cin;ty of nucleoli. The granules increased in number and were scattered throughout the nucleus. As infection progressed, the nucleolus became covere...

The development of giant cells induced by the nematode Meloidogyne in tomato roots has been followed under controlled growth conditions and the ultrastructure and histochemistry of these structures have been examined. Entry of the nematode larvae into the roots took place within 24 hours; giant cell formation started on the 4th day and involved breakdown of the cell walls accompanied by thicken...

introduction: the novel development of the (hba) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. the purpose of this study was to evaluate the correlation between hba score and histone deficiency, protamine content and dna fragmentation. methods: during april- march 2012 patients who were enrolled at shiraz-...

The structure of nucleic acid derived from thymus on the one hand and that derived from yeast on the other were finally established when it was shown by Levene and Jacobs (1) that the former was characterized by a pentose, dextroribose, whereas the other contained (Levene and London, 2) a reduced sugar, desoxyribose. A color reaction described by Feulgen (3) applicable to this sugar has been wi...

Tissue specimens from the cervical tumours of 70 patients undergoing radiotherapy were examined by Feulgen microdensitometry. Twenty-five of the 70 specimens were also subjected to in-vitro tritiated thymidine autoradiography to determine the proportion of DNA synthesising cells they contained. A spectrum of frequency distributions of nuclear DNA content was obtained from Feulgen microdensitome...

The identification and specific localization of chemical constituents within the cell nucleus have lagged considerably behind the qualitative and quantitative characterization of these classes of substances, with the possible exception of one component, the deoxyribonucleic acid (DNA). In addition to the latter, the nucleus contains ribonucleic acid1 2 (RNA), histone or basic protamine3 (BP), a...

The Feulgen reaction is a cytochemical test for the detection of desoxyribonucleic (tliymonucleic) acid. After acid hydrolysis, desoxyribonucleic acid in the presence of sulphurous acid gives a characteristic red-purple colour with basic fuchsin, and the depth of the colour roughly reflects the concentration of the compound. The detailed chemistry of the reaction has been fully described by Bak...