Accurate resolution of PCR products in the range of 15-40 kb may be obtained in agarose gels without pulsed field electrophoresis. A gel of 0.3% SeaKem Gold agarose cast on GelBond support film provides good resolution and sufficient get strength to reliably allow staining and photography. This paper describes a test system for Long PCR and demonstrates analysis of the PCR products on a gel run...

This paper presents a new method of precomputing highquality soft shadows that can be cast on a static scene as well as on dynamic objects added to that scene. The method efficiently merges the visibility computed from many shadow maps into a penumbra deep shadow map (PDSM) structure. The resulting structure effectively captures the changes of attenuation in each PDSM pixel, and therefore const...

background: creating a socket with proper fit is an important factor to ensure the comfort and control of prosthetic devices. several techniques are commonly used to cast transtibial stumps but their effect on stump shape deformation is not well understood. this study compares the dimensions, circumferences and volumes of the positive casts and also the socket comfort between two casting method...

background and aims. current chemical methods may not efficiently disinfect dental stone casts. the aim of this study was to investigate if microwave irradiation is effective for disinfection of stone casts. materials and methods. in this laboratory study, three groups (n = 162) of prepared spherical stone beads as carriers with a diameter of 10 mm were inoculated by separately soaking in three...

This paper presents an approach for educators to evaluate student progress throughout a course, and not merely based on a final exam. We introduce progress reports and describe how these can be used as a tool to evaluate student learning and understanding during programming courses. Complemented with data from surveys and the exam, the progress reports can be used to build an overall picture of...

The REDEEM authoring tool allows teachers to create adapted learning environments for their students from existing material. Previous evaluations have shown that under experimental conditions REDEEM can significantly improve learning. The goals of this study were twofold: to explore if REDEEM could improve students’ learning in real world situations and to examine if learners can share in the a...

Thin glass sheets may be manufactured using a two-part process in which a sheet is first cast and then subsequently reheated and drawn to a required thickness. The latter redrawing process typically results in a sheet with non-uniform thickness and with smaller width than the cast glass block. Experiments suggest that the loss of width can be minimized and the non-uniformities can be essentiall...