CONFORMATIONAL STABILITY OF HELICOBACTER PYLORI FLAVODOXIN. FIT TO FUNCTION AT pH 5. Nunilo Cremades, Marta Bueno, José Luis Neira, Adrián Velázquez-Campoy, & Javier Sancho Biocomputation and Complex Systems Physics Institute (BIFI), Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza. 50009 Zaragoza, Spain. Instituto de Biología Molecular y ...

ion at C-6 results in an allylic radical in which spin delocalization of the radical center makes it reactive with respect to the protein itself and thus results in covalent binding. b. Biotin Synthase is a Member of the RadicalSAM Family. Biotin synthase has been proposed to belong to the radical-SAM enzyme family for the following reasons.75 First it contains a (4Fe-4S)2+/1+ cluster required ...

CONFORMATIONAL STABILITY OF HELICOBACTER PYLORI FLAVODOXIN. FIT TO FUNCTION AT pH 5. Nunilo Cremades, Marta Bueno, José Luis Neira, Adrián Velázquez-Campoy, & Javier Sancho Biocomputation and Complex Systems Physics Institute (BIFI), Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza. 50009 Zaragoza, Spain. Instituto de Biología Molecular y ...

The nucleotide sequence of a 10,772 base pair (bp) region from Desulfovibrio gigas genome was determined. This sequence, which is adjacent to the region containing the coding units for the metalloproteins rubredoxin-oxygen oxidoreductase (ROO) and rubredoxin, includes the flavodoxin gene. Additionally, it also contains four open reading frames (ORFs) related to genes frequently found in replica...

8-Azidoflavins have been synthesized and their potential as photoaffinity labels for flavoproteins has been explored. They are very photolabile, and in aqueous media they react with solvent to yield 8-aminoflavins and 8-hydroxlaminoflavins as the main products. They fulfill the criteria expected of a good photoaffinity label, since they bind stoichiometrically at the flavin-binding site of flav...