A flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger beta-galactosidase in a continuous high-cell-density bioreactor. The delta-sequences from the yeast retrotransposon Ty1 were used as target sites for the integration of the beta-galactosidase expression cassette. High-copy-number transformants were successfully obtained using the delta-integration sy...

A flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger β-galactosidase in a continuous high-cell-density bioreactor. The δ-sequences from the yeast retrotransposon Ty1 were used as target sites for the integration of the β-galactosidase expression cassette. High-copy-number transformants were successfully obtained using the δ-integration system together ...

Spiral arm modulation in NGC 4062 (an optical flocculent) is detected for the first time; the Fourier spectra of NGC 4062 and NGC 5248 (a classic grand design in optical images) are almost identical. Within the framework of the Density Wave Theory (Lin and Shu 1964) and the the modal theory of galactic spiral structure (Bertin et al. 1989) the characteristic signature of a two-armed grand desig...

Super-Jupiters, brown dwarfs, and stars can form from the collapse of self-gravitating discs. Such discs are turbulent, with flocculent spiral arms accelerating gas to transonic speeds horizontally vertically. Objects that fragment gravito-turbulent should spin a wide range directions, reflecting random orientations their parent eddies. We show by direct numerical simulation obliquities newly c...

One in every three people the world still lacks access to clean drinking water. Aside from microbiological pollution, high fluoride content water is one of most serious problems African countries. This study aimed contribute availability by developing a point-of-use purifier using an aluminum oxide-based flocculent-disinfectant composite. Batch experiments were conducted determine factors affec...

We examined the mechanism by which hypertriglyceridemia interferes with the Roche Diagnostics "Amylochrome" procedure for measurement of amylase in lactescent samples with normal and above-normal amylase activity. The serum blank recommended in the Amylochrome protocol to compensate for lactescence remains inappropriately turbid, compared with the test, and is partly responsible for the underes...

During the Deepwater Horizon blowout, thick layers of oiled material were deposited on the deep seafloor. This large scale benthic concentration of oil is suggested to have occurred via the process of Marine Oil Snow Sedimentation and Flocculent Accumulation (MOSSFA). This meta-analysis investigates whether MOSSFA occurred in other large oil spills and identifies the main drivers of oil sedimen...

The purpose of this work was to develop a continuous fermentation system operating with a tower reactor using some flocculent yeast strains isolated from an industrial process. The strain was an used in the trial of the proposed system, composed of two serial glass tower reactor. The effects of the following variables were studied on the yield and productivity of the system: total reducing suga...

Streaming plasmodia of Physarum polycephalum were irradiated with a microscope-mounted ruby laser and the resulting changes were recorded by cinemicrography or streak photographs. Some lesions were processed for electron microscopy. By varying the incident energy, three levels of response were detected. Two transient responses, a gelation briefly blocking streams and a more severe gelation with...

Recent reports (1, 2, 3) that serum from febrile patients and exudates from tuberculous pleurae exert an apparently non-specific agglutinating effect upon certain gram-positive cocci have prompted the publication of similar results concerning the properties of exudates obtained from patients suffering from various acute manifestations of rheumatic fever. During the course of a search for agglut...