Dipeptidyl peptidase III (DPP III, EC 3.4.14.4) is a monozinc metalloexopeptidase that hydrolyzes dipeptides from the N-terminus of peptides consisting three or more amino acids. Recently, DPP has attracted great interest scientists, and numerous studies have been conducted showing it involved in regulation various physiological processes. Since only metalloenzyme among dipeptidyl peptidases, w...

High-quality mRNA extraction is essential for gene expression assays. In this study, we developed a rapid method (20 min) named FACTS the of intact RNA from Pseudomonas aeruginosa and compared its performance to established techniques like RNAsnap CiAR. The integrity, yield, purity presence residual genomic DNA were adopted as assessment criteria. Multiple assays integrity applied, including Ag...

Riboswitches reflect a novel concept in gene regulation that is particularly suited for technological adaptation. Therefore, we characterized thermodynamically the ligand binding properties of a synthetic, tetracycline (tc)-binding RNA aptamer, which regulates gene expression in a dose-dependent manner when inserted into the untranslated region of an mRNA. In vitro, one molecule of tc is bound ...