Background: CRISPR activation (CRISPRa) uses non-functional Cas9 endonuclease (dCas9) but retains the genome targeting ability through its single guide RNAs (sgRNAs). CRISPRa is widely used as a gene system exploiting in recruiting various transcriptional domains (TADs) for enhancing expression of target gene(s). Drought tolerant and resource-efficient crops like millets have potential mitigati...

Millets are renowned for their climatic resilience and possess high nutritive value with wide genetic variations. In countries like India Africa, millets part of many people's regular diets rich sources protein, dietary fiber, polyphenols, minerals, vitamins, other nutrients. The proteomic signatures several millet species, including Fonio, Finger, Proso, Sorghum, Foxtail millet, were examined ...

Objective: Fusarium diseases that cause grain mold and stalk rot of millet are the most important diseases, of these crops in Iran. Fusarium can cause stalk rots, ear rots, and grain mold, resulting in serious production losses in millet, and produce mycotoxins that are harmful to both humans and domesticated animals.  Methods: A total of 23 Fusarium isolates were rec...