Cucumisin (CUC) from industrial melon by-products was separated for the first time through biological precipitation using carrageenan (CRG). This approach could represent a cost-effective and environmentally friendly process industries, avoiding use of expensive equipment toxic salts or solvents, such as butanol ethanol. In this study, proteins CRG studied compared with conventional ammonium su...

A computational model for the analysis of lipoprotein distributions in the mouse: Translating FPLC profiles to lipoprotein metabolism Following parameter estimation and evaluation, two parameter sets were found to describe the data well (these will be referred to as sets X1 and X2). Inspection of the in silico FPLC profiles (e.g. Figure 1 in Text S4) reveals that in the VLDL and LDL size range,...

Among the microbial enzymes protease and amylase are most valuable which have been has diversified applications used extensively because of their capabilities in degradation organic wastes, application biofuels, agricultural, pharmaceuticals, chemical biotechnological industries. The aim current research work was purification, characterization alkaline proteases extracted from Bacillus cereus A...

5 Abstract The bacterial strains Rhodococcus (opacus) erythropolis HL PM-1 and Nocardioides simplex FJ2-1A can use picric acid (TNP) and 2,4-dinitrophenol (DNP) as sole nitrogen source. Enzymes were identified which are isofunctional in both strains, using the same degradation mechanism. During the initial steps hydride ions were transferred to the aromatic ring of the electron deficient π-syst...

Disturbances of lipoprotein metabolism are recognized as indicators of cardiometabolic disease risk. Lipoprotein size and composition, measured in a lipoprotein profile, are considered to be disease risk markers. However, the measured profile is a collective result of complex metabolic interactions, which complicates the identification of changes in metabolism. In this study we aim to develop a...

Background: Lactoferrin (Lf) is a glycoprotein, a member of the transferrin family.From ten known mechanisms of anti-cancer chemoperotecive compounds, Lf alone, has six of these functions and inhibits cancer. In this study, the effect of lactoferrin purified from bovine colostrum was studied as an anti-cancer agent on esophageal cancer cell line. Materials and Methods: Bovine colostrum were ...

This study was performed in order to define the cellular immune response of 12 recovered cutaneous leishmaniasis subjects to different soluble antigens of the amastigote form of Leishmania major. A soluble leishmanial antigen preparation (SLA) derived from highly-purified amastigotes from infected nude mice was fractionated by Mono Q column using Fast Protein Liquid Chromatography (FPLC) s...

Neuropeptide Y (NPY) is a 36 amino acid peptide found throughout the central and peripheral nervoussystem of rat and human. NPY has been proposed to play an important role in satiety. The aim of this studywas to produce cell lines that secrete high levels of bioactive NPY. For this purpose, the complementaryDNA (cDNA) that encodes NPY was isolated by PCR. The cDNA was then clo...

introduction: mcp (membrane cofactor protein or cd46) is a complement regulatory proteins (crps), which is present in most of body cell membranes (except erythrocytes), and also in most biologycal body fliuds. due to the important role of this protein in the complement system, specially in fertility, the aim of this study was to determine and isolate this protein in human seminal plasma. materi...

It has been reported that the activation of dihydrofolate reductase (DHFR) from L1210 mouse leukaemia cells by KCl or thiol modifiers is accompanied by increased digestibility by proteinases [Duffy, Beckman, Peterson, Vitols and Huennekens (1987) J. Biol. Chem. 262, 7028-7033], suggesting a loosening up of the general compact structure of the enzyme. In the present study, the peptide fragments ...