The parameters that affect the interaction of ligands with a fucose-binding lectin from rat liver have been examined. 125I-Fucosyl-bovine serum albumin (Fuc-BSA) containing 50 residues of fucose/molecule was used as the standard ligand. At low initial concentrations of ligand (10 ng/ml) and lectin (140 ng/ml), the reaction reaches equilibrium at pH 7.8, 23 degrees C, within 40 min. The binding ...

Concentrated faecal extracts from breast and artificially fed infants during the first week of life were examined by paper chromatography. Besides lactose, glucose and galactose, five oligosaccharide spots were detected. Breast-fed infants showed an additional fucose spot. Complete hydrolysis of the individual oligosaccharides showed glucose and galactose always and fucose in two instances. The...

‡ Department für Chemie, Universität für Bodenkultur, A-1190 Wien, Austria § Institut für Mikrobiologie, ETH Zürich, Wolfgang-Pauli-Str. 10, 8093 Zürich, Switzerland ¶ Institut für Parasitologie and || Vetomics Core Facility for Research, Veterinärmedizinische Universität, A-1210 Wien, Austria ** Department of Chromosome Biology, Max F. Perutz Laboratories, Universität Wien, A-1030 Wien, Austri...

Objective: Detecting the level of antioxidant and tissue damage that can occur in liver induced metabolic syndrome by a high fructose diet rats changes after exercise and/or caloric restriction. Materials Methods: Sprague-Dawley male were divided into five groups: control (C), (M), with (ME), restriction (MCR), (MECR). To induce syndrome, 10% solution was given to drinking water for 3 months. E...

A novel fucose-binding lectin, designated SauFBP32, was purified by affinity chromatography on fucose-agarose, from the serum of the gilt head bream Sparus aurata. Electrophoretic mobility of the subunit revealed apparent molecular weights of 35 and 30 kDa under reducing and non-reducing conditions, respectively. Size exclusion analysis suggests that the native lectin is a monomer under the sel...

Lectin microarray technology was applied to compare the glycosylation pattern of the monoclonal antibody MB311 expressed in SP2.0 cells to an antibody-dependent cellular cytotoxic effector function (ADCC)-optimized variant (MB314). MB314 was generated by a plant expression system that uses genetically modified moss protoplasts (Physcomitrella patens) to generate a de-fucosylated version of MB31...

The number of goblet cells in the small intestine decreased in the vitamin A-deficient rat. Incorporation of W-D-glucosamine into a fucose-containing glycopeptide declined markedly. Ultracentrifugation of this glycopeptide in Hz0 gave only a single component, with a sedimentation value of 3.6 S, whereas, in 0.1 M phosphate buffer (pH 7.5) two components appeared, with much higher sedimentation ...

Core fucose-specific lectin from Pholiota squarrosa 1 A novel core fucose-specific lectin from the mushroom Pholiota squarrosa* Background: Fuc-α1-6 oligosaccharide has a variety of biological functions. Results: Purification of a novel Fucα 1-6-specific lectin from the mushroom Pholiota squarrosa. Conclusion: The lectin binds only to core α1-6 fucosylated N-glycans and not to the other types o...

Fucosylated chondroitin sulfate (FuCS), a kind of complex glycosaminoglycan from sea cucumber, has potent anticoagulant activity. In order to understand the relationship between structures and activity, the depolymerized FuCS (dFuCS) was chosen to prepare its derivates by selective substitution at OH groups. Its O-acylation was carried out in a homogeneous way using carboxylic acid anhydrides. ...

Brown alga Fucus evanescens, widespread in the Far Eastern seas of Russia, is valuable source of sulfated polysaccharides—fucoidans with beneficial biological activities. The most homogenous fraction of fucoidan from F. evanescens was shown to be molecule containing linear main chain of alternating 2-sulfated 1,3and 1,4-linked α-L-fucose residues. Few sulfate groups were found in position 4 of ...