Loss of glycosaminoglycan (GAG) chains of proteoglycans (PGs) is an early event of osteoarthritis (OA) resulting in cartilage degradation that has been previously demonstrated in both huma and experimental OA models. However, the mechanism of GAG loss and the role of xylosyltransferase-I (XT-I) that initiates GAG biosynthesis onto PG molecules in the pathogenic process of human OA are unknown. ...

OBJECTIVE The aim of this study was to examine the course of time-dependent evolution of HIV-1 subtype A on a global level, especially with respect to the dynamics of immunogenic HIV gag epitopes. METHODS We used a total of 1,893 HIV-1 subtype A gag sequences representing a timeline from 1985 through 2010, and 19 different countries in Africa, Europe and Asia. The phylogenetic relationship of...

Objective(s): Mucopolysaccharidosis VI (MPS VI) or Maroteaux-Lamy syndrome is a rare metabolic disorder, resulting from the deficient activity of the lysosomal enzyme arylsulfatase B (ARSB).  The enzymatic defect of ARSB leads to progressive lysosomal storage disorder and accumulation of glycosaminoglycan (GAG) dermatan sulfate (DS), which causes harmful effects on various organs and tissues an...

Introduction: Failure of human body tissue and organs is believed to be one of the most important health problems all over the world. The great challenge for tissue engineers is to optimize suitable systems to separate, proliferate and differentiate the cells so that they can set out to create tissue by a harmonic 3-D growth. Therefore, the tissue engineers must provide an environment like the ...

To explore the possibility of using gene transfer to provide iduronate-2-sulfatase (IDS; EC 3.1.6.13) enzyme activity for treatment of Hunter syndrome, an amphotropic retroviral vector, L2SN, containing the human IDS coding sequence was constructed and studied for gene expression in vitro. Lymphoblastoid cell lines (LCLs) from patients with Hunter syndrome were transduced with L2SN and expresse...

Does CyP play a role in HIV-1 replication? CyPA, the member of the CyP family that colocalizes with Gag in College of Physicians and Surgeons 701 West 168th Street the cytoplasm, is specifically incorporated into HIV-1 virions via interaction with the Gag polyprotein Gag binding to CyPA requires a proline-rich region located in the center of the CA domain, and mutation of a single proline, P90,...

objective: cartilage mass produced from mesenchymal stem cell (msc) differentiation would be a suitable candidate for use in regenerative medicine. since the proper function of cartilage tissue is largely dependent on matrix glycosaminoglycan (gag) contents, the objective of this study was to investigate the enhancing effect of two gsk3 inhibitors on the gag content of cartilage produced by hum...