This document provides the latest recommendations for evaluation of potential sperm, oocyte, and embryo donors as well their recipients, incorporating recent information about optimal screening testing sexually transmitted infections, genetic diseases, psychological assessments. revised incorporates from US Centers Disease Control Prevention, Food Drug Administration, American Association Tissu...

BACKGROUND Currently, even the most sophisticated methods of assisted reproductive technology (ART) allow us to achieve live births in only approximately 30% of patients, indicating that our understanding of the fine mechanisms underlying reproduction is far from ideal. One of the main challenges associated with studies of gamete structure and function is that these cells are remarkably resista...

The release of eggs and sperm into the environment for external fertilization is common (Giese & Kanatani 1987) and thought to be the ancestral mating strategy (Wray 1995). Most phyla have at least some species that release sperm into the environment (free spawning or spermcasting) and a majority have representatives that also release eggs (broadcast spawning) for external fertilization (Levita...

The production of transgenic animals is an important tool for experimental and applied biology. Over the years, many approaches for the production of transgenic animals have been tried, including pronuclear microinjection, sperm-mediated gene transfer, transfection of male germ cells, somatic cell nuclear transfer and the use of lentiviral vectors. In the present study, we developed a new trans...