Temozolomide (TMZ) is approved for use as first-line treatment for glioblastoma multiforme (GBM). However, GBM shows chemoresistance shortly after the initiation of treatment. In order to detect whether silencing of human protein phosphatase 1D magnesium dependent (PPM1D) gene could increase the effects of TMZ in glioma cells, glioma cells U87-MG were infected with lentiviral shRNA vector targe...

The leafhopper Euscelidius variegatus is a natural vector of the chrysanthemum yellows phytoplasma (CYp) and laboratory Flavescence dorée (FDp). Previous studies indicated crucial role for insect ATP synthase α β subunits during infection species. Gene silencing was obtained by injection specific dsRNAs in E. variegatus. Here we present long-lasting nature such silencing, its effects on small R...

Y‐satellite RNA (Y‐sat) of cucumber mosaic virus upregulates the expression aphid ABCG4 gene, which promotes wing formation. We used virus‐induced gene silencing (VIGS) to prevent wing‐induction mechanism Y‐sat and thus inhibited Of aphids on plants with VIGS , only about 30% had wings, 60–70% winged were small likely impaired in flying ability. In addition, we showed that double‐stranded RNAs ...