Accurate, economical and high-throughput gene and genome synthesis is essential to the development of synthetic biology and biotechnology. New large-scale gene synthesis methods harnessing the power of DNA microchips have recently been demonstrated. Yet, the technology is still compromised by a high occurrence of errors in the synthesized products. These errors still require substantial effort ...

In the present study, using anti-sense oligonucleotides the inhibition of expression of the PML protein hasbeen investigated. The anti-sense oligonucleotides were designed against the translation initiation site ofthe PML gene, and their effects were investigated on cellular growth and DNA synthesis. Incubation of normalhuman fibroblast cells with the anti-sense oligonucleotid...

Translesion synthesis (TLS) employs low fidelity polymerases to replicate past damaged DNA in a potentially error-prone process. Regulatory mechanisms that prevent TLS-associated mutagenesis are unknown; however, our recent studies suggest that the PCNA-binding protein Spartan plays a role in suppression of damage-induced mutagenesis. Here, we show that Spartan negatively regulates error-prone ...

This paper presents a demonstration of a stochastic prosody tool for enrichment of synthesized speech using SSML prosody tags applied over hierarchical thematicity spans in the context of a CTS application. The motivation for using hierarchical thematicity is exemplified, together with the capabilities of the module to generate a variety of SSML prosody tags within a controlled range of values ...

Regulation of gene expression levels is essential for all living systems and transcription factors (TFs) are the main regulators of gene expression through their ability to repress or induce transcription. A balance between synthesis and degradation rates controls gene expression levels. To determine which rate is dominant, we analyzed the correlation between expression levels of a TF and its r...

conclusions our findings revealed that the zfn technology could be employed to overcome ampicillin resistance by the targeted disruption of the ampicillin resistance gene, which leads to inactivation of β-lactam synthesis. therefore, zfn technology could be engaged to decrease the antibiotic resistance issue with the construction of a zfn archive against different args. to tackle the resistance...

Estrogen and iron deficiency regulate the synthesis of transferrin in chick liver by causing a specific increase in transcription of the transferrin gene. The relative rate of synthesis of transferrin mRNA was measured by allowing isolated nuclei to continue transcription in vitro in the presence of [CU-~~P]UTP. The 32P transcripts then were hybridized to immobilized recombinant DNA containing ...

Introduction: Astrocytes, the most abundant glia in the central nervous system, modulate neuronal survival and function. Astrocytic functions are mediated by synthesis and secretion of wide ranges of polypeptides through mechanism (s) poorly understood. Among these, TGFβs are synthesized and released by the astrocytes. In this study, the involvement of Wnt signaling pathway on the synthesi...

Estrogen and iron deficiency regulate the synthesis of transferrin in chick liver by causing a specific increase in transcription of the transferrin gene. The relative rate of synthesis of transferrin mRNA was measured by allowing isolated nuclei to continue transcription in vitro in the presence of [CU-~~P]UTP. The 32P transcripts then were hybridized to immobilized recombinant DNA containing ...

BACKGROUND Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenesis procedures and allows generating nucleic acids for which no template is available. In addition, when coupled with efficient gene design algorithms that optimize codon usag...