P22-mediated generalized transduction in S. typhimwiwn occurs in both sensitive and lysogenic recipient bacteria, but the efficiency of transduction is significantly higherinsensitivecells t.han in wild-type P22 lysogens. This reduction in the efficiency of transduction is under the control of the prophage sieA gene; this gene is also responsible for the exclusion of superinfect,ing phage. Expe...

Gonadotropin-releasing hormone (GnRH) binds to the pituitary GnRH receptor to activate signal transduction cascades that ultimately modulate gonadotropin biosynthesis. Comprehensive studies of the GnRH-activated gene program in the LbetaT2 gonadotrope cell line have greatly increased our knowledge of the number of early and intermediate gene transcripts that are modulated by GnRH. Among the cla...

Intra-articular gene therapy has potential for the treatment of osteoarthritis and rheumatoid arthritis. To quantify in vitro relative gene transduction, equine chondrocytes and synovial cells were treated with adenovirus vectors (Ad), serotype 2 adeno-associated virus vectors (rAAV2), or self-complementary (sc) AAV2 vectors carrying green fluorescent protein (GFP). Using 6 horses, bilateral me...

Feline leukemia virus (FeLV) induces neoplastic and nonneoplastic diseases in cats. The transduction of cellular genes by FeLV is sometimes observed and associated with neoplastic diseases including lymphoma and sarcoma. Here, we report the first natural case of feline Notch2 transduction by FeLV in an infected cat with multicentric lymphoma and hypercalcemia. We cloned recombinant FeLVs harbor...

Signal transduction pathways control most cellular activities in living cells ranging from regulation of gene expression to fine-tuning enzymatic activity and controlling motile behavior in response to extracellular and intracellular signals. Because of their extreme sequence variability and extensive domain shuffling, signal transduction proteins are difficult to identify, and their current an...

Adenoviral gene transfer to primitive hematopoietic progenitor cells (HPCs) would be useful in gene therapy applications where transient, high-level transgene expression is required. In the present investigations, we have used an adenoviral vector expressing the green fluorescent protein (AdGFP) to quantify transduction of primitive HPCs and assess adenoviral-associated toxicity in long-term cu...

Koalas suffer unusually high rates of neoplasia. There has been a long-standing correlation between koalas with the koala retrovirus (KoRV) and development neoplasia which lacked mechanistic explanation. We describe recent results that demonstrate many KoRV integrations lead to by (I) inserting into somatic cells preferentially near oncogenes, (II) germ oncogenes predisposing cancer, (III) tran...