Quorum sensing (QS) is a cell density-dependent mechanism used by many pathogenic bacteria for regulating virulence gene expression. Inhibition or interruption of QS by medicinal plant remedies has been suggested as a new strategy for fighting against antibiotic-resistant bacteria. This study aimed to assess the impact of sub-inhibitory concentrations of licochalcone A (LAA) and epigallocatechi...

Background: In gene therapy, the use of RNA molecules as therapeutic agents has shown advantages over plasmid DNA, including higher levels of safety. However, transient nature of RNA has been a major obstacle in application of RNA in gene therapy. Methods: Here, we used the internal ribosomal entry site of encephalomyocarditis virus and the 3’ non-translated region of Poliovirus to design an en...

S. rebaudiana produces steviol glycosides including stevioside and rebaudioside A that are valuable as low calorie sweeteners. The objective of this study was to investigate the effects of salicylic acid elicitation and sampling times on the improvment of stevioside and rebaudioside A production and KA13H, UGT74G1 and UGT76G1 genes expression. The results showed that the addition of different c...

Background: Experimental cryptorchidism is a common model for examining the expression and function of heat-induced spermatogenesis related genes in testis. In this study the expression of genes & proteins thatinvolved in programmed cell death after treatment of cryptorchidism was examined. Materials and Methods: To induce bilateral cryptorchid model immature mice were anesthetized and a small ...

Tomato is one of the important plants in the world as a food, medicine etc. it is sensitive to abiotic stresses and its crop is affected by them. Moreover, Ascorbic acid (AsA) is one of the universal of plant defence mechanism against the stresses specially salinity and drought to scavenger’s reactive oxygen species. According to global warming and scarcity of water resources, subsequently dryi...

Background: To screen and diagnose patients with recurrent abortions and implantation failure after IVF/ICSI, differentially expressed genes of endometrium through DNA microarrays were monitored. Materials and Methods: Microarray expression profile of GSE26787 dataset from GEO database was used to analyze gene expression profiles of 15 endometrial biopsy samples- five from control fertile (CF) ...

objective: this study was designed to evaluate the effects of vitrification and in vitro culture of human ovarian tissue on the expression of oocytic and follicular cell-related genes. materials and methods: in this comparative study, ovarian tissue samples were obtained from eight transsexual women. samples were cut into small fragments and were then assigned to vitrified and non-vitrified gro...

There are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. Bicistronic system has disadvantages e.g. compared with two-promoter system. In this paper, a simple method based on spliced overlap extension by polymerase chain reaction (SOE-PCR) technique was demonstrat...