Mitochondrial DNA (mtDNA) accumulates both base-substitution mutations and deletions with aging in several tissues in mammals. Here, we examine the evidence supporting a causative role for mtDNA mutations in mammalian aging. We describe and compare human diseases and mouse models associated with mitochondrial genome instability. We also discuss potential mechanisms for the generation of these m...

Vanadium (V) has become a serious pollutant due to its widespread use in industry. In this study, DNA methylation changes Triticum aestivum L. 'Çetinel 2000' seeds were determined by CRED-RA (Coupled Restriction Enzyme Digestion-Random Amplification) analysis. damage and genomic instability Simple Repeat Inter-Sequence Polymorphism (ISSR) solutions (4.4 mM, 6.6 8.8 mM) boric acid (BA) (4 mM 8 u...

In the present study, amaranth, which is a very important azo dye used in food, drug, paper, cosmetic and textile industries, was evaluated for its genotoxic potential by plant model organism Triticum aestivum (wheat). The impacts of salicylic acid on damage, alterations DNA methylation due to amaranth stress L. were also investigated. marker techniques Inter-retrotransposon amplified polymorph...

cyp3a4 probably has the broadest catalytic activity of any cytochrome p450. it is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. firstly, a total of 300 bp core distal enhancer of cyp3a4 xrem region (-7972/-7673) were amplified from human genomic dna. the pcr product was then ligated into a human secretory alkaline phosphat...

There is wide-spread interest in understanding the rate of transposable element movement within populations and between species. A recent study using interprecific crosses between D. buzzatii and D. koepferae indicated that transposition rates in hybrids may be quite high. However, we suggest caution should be taken in this interpretation since AFLP methods to detect transposition events may le...

R-loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R-loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic rep...

Full genome sequencing in higher plants is a very difficult task, because their genomes are often very large and repetitive. For this reason, gene targeted partial genomic sequencing becomes a realistic option. The method reported here is a simple approach to generate gene-enriched plant genomic libraries called methylation filtration. This technique takes advantage of the fact that repetitive ...

BACKGROUND Abnormal one-carbon metabolism may lead to general genomic (global) hypomethylation, which may predispose an individual to the development of colorectal neoplasia. METHODS We evaluated the association between pre-diagnostic leukocyte genomic DNA methylation level and the risk of colorectal cancer in a nested case-control study of 358 colorectal cancer cases and 661 matched controls...

The ability to amplify genomic DNA in a polymerase chain reaction (PCR) is dependent upon the purity of the DNA template. Environmental genomic DNA often contains contaminants (e.g., polyphenols, humic acids, polysaccharides) that reduce template purity and can be difficult to remove, thereby inhibiting PCR amplification. There is thus a need for a method to purify extracted genomic DNA without...

dermatophytes are a group of keratinophilic fungi capable of invading keratinized tissues (skin, hair and nails). they cause dermatophytosis (commonly known as tinea or ring worm) in human and animals. in this report, dna similarities and genomic linkage of 40 dermatophytes strains was obtained from different universities, were studied by random amplified polymorphic dna (rapd–pcr) using 11 ran...