Spores of Geobacillus stearothermophilus at two inoculum levels (3 and 6 log colony forming units per gram, cfu/g) were challenged with superheated steam (SS) at a velocity of 0.35 m/s and temperature of 130, 145, 160, and 175C. Tailing was observed in the survivor curves (plots of survival ratios, log( o N N ) against treatment time) as a monotonic upward concavity. Therefore, a non-linear reg...

Azoreductases have been characterized as enzymes that can decolorize azo dyes by reducing azo groups. In this study, genes encoding proteins having homology with the azoreductase gene of Bacillus sp. OY1-2 were obtained from Bacillus subtilis ATCC6633, B. subtilis ISW1214, and Geobacillus stearotherophilus IFO13737 by polymerase chain reaction. All three genes encoded proteins with 174 amino ac...

Geobacillus stearothermophilus T-6 utilizes an extensive and highly regulated hemicellulolytic system. The genes comprising the xylanolytic system are clustered in a 39.7-kb chromosomal segment. This segment contains a 6-kb transcriptional unit (xynDCEFG) coding for a potential two-component system (xynDC) and an ATP-binding cassette (ABC) transport system (xynEFG). The xynD promoter region con...

Here, we present an immobilized enzyme cascade in a basket-type reactor allowing one-pot, two-step enzymatic synthesis of L-erythrulose from D-serine and glycolaldehyde. Three enzymes, D-amino acid oxidase Rhodotorula gracilis (DAAORg), catalase bovine liver (CAT), transketolase Geobacillus stearothermophilus (TKgst) were covalently on silica monolithic pellets, characterized by open structure ...

Glycosynthases are catalytic mutants of mainly retaining glycoside hydrolases that catalyze the synthesis of oligosaccharides from their corresponding glycosyl-fluoride donors and suitable acceptors. Here we describe the development of a general, high-throughput screening procedure for glycosynthase activity, which is based on the release of hydrofluoric acid, a by-product of all glycosynthase ...