Microcosm studies were performed to evaluate the effect of solid surfaces, bacterial adhesive ability, and inoculum size on colonization success and persistence of Pseudomonas fluorescens and Xanthomonas maltophilia, each with a Tn5 insertion that conferred resistance to kanamycin and streptomycin. Two types of microcosms were used: (i) a simple system that was colonized by Aeromonas hydrophila...

We have found that agitation of Saccharomyces cerevisiae with glass beads and plasmid DNA using a vortex mixer results in genetic transformation of the yeast cells. This method is less efficient, but considerably more convenient, than other yeast transformation procedures. The fact that the minimal requirements for transformation are simply physical damage and the presence of DNA in an osmotica...

Self-assembled monolayers (SAMs) are monomolecular layers which are spontaneously formed upon immersing a solid substrate into a solution containing functional molecules. The most attractive feature of SAMs is molecular level control over the modification of surfaces, and incorporation of multiple or multilayer molecular components onto the monolayer leads to various functional properties. SAMs...

A total of seventeen annular transparent blue glass beads and one cylindrical bead with opaque grey-white decoration were found at a site near Stotfold, in Bedfordshire, England. The part cremation burial (without an urn), associated small fragments gold sheet bone, the latter carbon dated to 1303-1052 calBC 95% probability. Analysis (quantitative using SEM-EDS qualitative XRF) that are made fr...

We investigated the organization of micrometric hydrophilic beads (glass or basalt) immersed in Leidenfrost drops. Starting from a large volume of water compared to the volume of the beads, while the liquid evaporates, we observed that the grains are eventually trapped at the interface of the droplet and accumulate. At a moment, the grains entirely cover the droplet. We measured the surface are...

A partially purified oxalate oxidase from leaves of 10-day-old seedlings of forage sorghum was immobilized covalently onto alkylamine glass beads affixed on inner base of a glass beaker. The enzyme retained 5.17% of its initial activity with a conjugation yield of 182 mg/g support. After immobilization, the enzyme showed an increase in its optimum pH and Km for oxalate but slight decrease in in...

The glass ingots from the Late Bronze Age Uluburun shipwreck (ca. 1300 BCE) provide crucial insight into production and exchange in Mediterranean. Almost all of approximately 200 on board ship were sampled as well five 30 Mycenaean relief beads. Here we report full chemical compositional results for these samples, along with 49 strontium isotope analyses representing at least 48 separate ingots...

Background: Chronic osteomyelitis is a disease usually of infectious origin. The main cause post-traumatic, it affects the bone tissue and surrounding tissue, most frequent causative agent Staphylococcus aureus. affected tibia. Case description: A 42-year-old male with diagnosis chronic tibia osteomyelitis, sequelae previous surgical interventions, multiple antibiotic treatments, ...