A vast excess of α-globin production and inadequate γ-globin compensation lead to the development of severe anemia in human β-thalassemia. Newly identified modifiers of αand γ-globin synthesis and insights into the mechanisms of globin regulation provide the tools for potential new approaches to treating this and other red blood cell disorders. In the study by Han and colleagues in this issue o...

Evidence is presented that indicates that greater than 95 % of the protein synthesized by intact rabbit reticulocytes is globin and that only globin nascent chains are found on the membrane-bound ribosomes from reticulocytes. This result conflicts with reports in the literature that approximately 14% of the protein synthesized by reticulocytes is non-globin and that 86% of the protein synthesiz...

We have constructed fusion genes comprised of y and p globin elements and globin sequences linked to neomycin resistance (neon) genes to define the cis acting sequences responsible for developmental stage-specific expression and induction of fetal globin genes in embryonic-fetal erythroleukemia K562 cells. The results indicate that the y promoter is required for proper initiation of transcripti...

The presence of minute amounts of embryonic -globin chains in adult hemolysates is a marker for carriers of a-thalassemia-1 resulting from ( / ) deletion. Recently. we developed a murine monoclonal antihuman embryonic -globin chain antibody, 8E8. By using this antibody. we have now established a slot-blot immunobinding assay for the rapid detection of -globin chains in adult hemolysates. -globi...

We have isolated the delta-globin gene of the New-World spider monkey, Ateles geoffroyi, and compared its nucleotide sequence with those of other primate delta- and beta-globin genes. Among primate delta-globin genes, the rate of nonsynonymous substitutions is much less than the rate of synonymous substitutions. This suggests that primate delta-globin genes may remain under evolutionary conserv...

Background: β-thalassemia is one of the most widespread disease in the world, including Iran. In this study, we reported, for the first time, A 290-bp β-globin gene deletion in the south of Iran. Methods: Four individuals from three unrelated families with Arabic ethnic background were studied in Khuzestan Province. Red blood cell indices and hemoglobin analysis were carried out accor...

products and tryptic digestion and ‘mapping’ of the peptides (Sargent & Vadlamudi, 1968; Mainwaring, 1964), indicated that globin was a product of the wheat-germ system. This globin was shown to be a mixture of the embryonic P and E globins by use of [3H]globin markers in the peptide ‘mapping’. It appears from our findings that a t 12h of incubation in uitro the mRNA for globin is present, but ...

We have set up an experimental system to map the primary transcription unit of the human alpha 2 globin gene. The duplicated human alpha globin genes (alpha 2-alpha 1) were linked to the alpha globin locus Positive Regulatory Element (PRE) and stably transfected into murine erythroleukaemia cells. We then developed a quantitative reverse transcriptase, polymerase chain reaction assay to map alp...

In two black families with the hereditary persistence of fetal hemoglobin (HPFH) gene there are eight A-F heterozygotes and two double heterozygotes for sickle cell trait and HPFH. These patients are clinically asymptomatic and have homogeneous acid elution smears. Measurement of globin chain synthesis in peripheral blood demonstrates balanced production of a alpha and non-alpha (beta plus gamm...

We have investigated the DNase I hypersensitivity of a hybrid mouse/human beta-globin gene in erythroid and non-erythroid cells of transgenic mice, to examine the relationship between the chromatin structure and the expression of an exogenous gene. The hybrid globin gene was previously shown to be expressed specifically in erythroid cells in some transgenic lines. The maximal level of hybrid gl...