The glutamic acid E396, aspartic acid D409 and glutamic acid E411 residues of the Escherichia coli penicillin-binding protein 3 were each converted into an alanine residue. As deduced from penicillin-binding and complementation experiments, none of these dicarboxylic acid residues is involved in the mechanism of acylation by penicillin and none of them is essential for the in vivo functioning o...

Previous studies (1) of glutamic acid metabolism suggested an investigation of the role of this amino acid and its thio esters (2, 3) in amino acid incorporation. The studies by Zamecnik et al. (4, 5) on amino acid incorporation into freshly prepared rat liver microsomes in the presence of the soluble cell fraction and an ATPl-regenerating system appeared to provide a suitable test system for o...

Previous studies (1) of glutamic acid metabolism suggested an investigation of the role of this amino acid and its thio esters (2, 3) in amino acid incorporation. The studies by Zamecnik et al. (4, 5) on amino acid incorporation into freshly prepared rat liver microsomes in the presence of the soluble cell fraction and an ATPl-regenerating system appeared to provide a suitable test system for o...

The influence of organic additives, β-cyclodextrin, HEDTA, L-aspartic acid and L-glutamic acid on crystal growth of calcium carbonate via vapour diffusion method has been investigated. In the absence of additive, regular rhombohedral calcium carbonate crystal habit resulted. Whereas in presence of additive, calcite with truncated, aggregated rhombohedral sub crystals and elongated hexagonal mor...

In contrast to fi-glutamine and @glutamylhydroxamic acid, fl-glutamic acid does not possess an asymmetric carbon atom, but has, according to the nomenclature of Schwartz and Carter (2), a meso carbon atom. The present experiments were designed to determine whether glutamine synthetase distinguishes between the carboxyl groups of P-glutamic acid. It is noteworthy in this connection that the enzy...

Proline is synthesized from glutamic acid by way of the intermediate glutamic -y-semialdehyde, as in the following equation: glutamic acid + glutamic 'y-semialdehyde -* proline. In Escherichia coli W, the first reaction is subject to endproduct inhibition by proline (A. Baich and D. J. Pierson, Biochim. Biophys. Acta 104:397, 1965). This reaction is also inhibited by low, although higher than b...

Greenhouse grown seedlings of corn (Zea mays L.) and foxtail (Setaria faberii Herrm.) were used as source material in determining the intracellular localization of nitrate reductase, nitrite reductase, and glutamic acid dehydrogenase, Nonaqueous and aqueous isolation techniques were used to establish that nitrite reductase is localized within the chloroplasts, but that nitrate reductase and glu...

In order to further improve the biocompatibility of xylitol based poly(xylitol sebacate) (PXS) bioelastomer, a novel kind of amino acid based poly(xylitol glutamate sebacate) (PXGS) has been successfully prepared in this work by melt polycondensation of xylitol, N-Boc glutamic acid and sebacic acid. Differential scanning calorimetry (DSC) results indicated the glass-transition temperatures coul...