Copyright © 2005 American Heart Association. All rights reserved. Print ISSN: 0009-7322. Online 72514 Circulation is published by the American Heart Association. 7272 Greenville Avenue, Dallas, TX DOI: 10.1161/CIRCULATIONAHA.105.571919 2005;112;2078-2080 Circulation Michele Emdin, Alfonso Pompella and Aldo Paolicchi Triggering Oxidative Stress Within the Plaque Gamma-Glutamyltransferase, Athero...

Brush border γ-glutamyltransferase-glutaminase activity and the high-affinity glutamate transporter EAAC1 function as a unit in generating and transporting extracellular glutamate into proximal tubules as a signal that modulates intracellular glutamine/glutamate metabolism, paracellular permeability, and urinary acidification. The reported presence of a second glutamate transporter, GLT1, on th...

Intact rat liver cells from the perivenous region were isolated by collagenase perfusion after first destroying the periportal region by a brief portal infusion of digitonin. Periportal cells were isolated after retrograde digitonin infusion. Significantly higher alanine aminotransferase, gamma-glutamyltransferase and lactate dehydrogenase activities and lower glutamate dehydrogenase and pyruva...

Neuronal microtubules support intracellular transport, facilitate axon growth, and form a basis for neuronal morphology. While microtubules in nonneuronal cells are depolymerized by cold, Ca(2+), or antimitotic drugs, neuronal microtubules are unusually stable. Such stability is important for normal axon growth and maintenance, while hyperstability may compromise neuronal function in aging and ...

Clinical Enzymology Enzymes nomenclature, classification, structure and specificity Enzymes, isoenzymes and their relevance in diagnosis Regulation of enzyme levels in serum and plasma Selection of enzyme tests Measurement of isoenzymes and isoforms Some important enzymes in clinical practice Aspartate transaminase (AST) and Alanine transaminase (ALT) Creatine Kinase (CK) Lactate dehydrogenase ...

H epatotoxicity is an often reported side effect of azathioprine treatment, which requires monitoring of serum transaminases and c-glutamyltransferase (cGT), especially during the first 6 months of treatment. This case report, however, illustrates that side effects of azathioprine can also occur after a longer period of treatment. We report a case of sinusoidal dilatation as a rare side effect ...

We describe the preparation of a new substrate, N-gamma-L-glutamyl-5-aminoisophthalic acid dimethyl ester hydro-chloride, for the fluorometric determination of gamma-glutamyltransferase activity in serum by the "front-surface" technique. Details of the resulting method are provided. The final reaction mixture contains 4 mmol of the substrate per liter of tris(hydroxymethyl)aminomethane (100 mmo...

We have studied the activity of a calcium dependent transglutaminase (EC 2.3.2.13) during the growth of the parasite Plasmodium falciparum inside the infected human erythrocyte. There is only one detectable transglutaminase in the two-cell-system, and its origin is erythrocytic. No activity was detected in preparations of the parasite devoid of erythrocyte cytoplasm. The Michaelis Menten consta...

In a previous publication (1) methods were described which permit the quantitative estimation of the action of y-glutamyl transpeptidase upon glutathione. In the present report these methods are employed to study the influence of amino acids. pcptides, and other compounds upon the reaction. In addition, we present the results of attempts to account for all the main products formed in the reacti...

Gamma-Glutamyltransferase activity was studied in a man presenting with recurrent septicemia owing to pyonephrosis and renal carcinoma. Increased activity in the urine was ascribable to administration of the aminoglycoside antibiotic, tobramycin. That the renal carcinoma did not contribute to the increased values was confirmed by homogenization and enzyme histochemistry of the tumor. Although t...