Silver is back in vogue today as this metal used the form of nanomaterials numerous commercial products. We have developed in-gel electrophoretic techniques to measure activity antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX), same combination with HSP70 Western blot analysis evaluate effects nanomolar amounts silver nitrate 5 nm alkane-coated nan...

Non Insulin Dependent Diabetes Mellitus is responsible for 60% cases of retinopathy in the population and is one of the common cause of blindness. Oxidative stress as measured by the levels of malondialdehyde, superoxide dismutase (SOD), glutathione peroxidase (GPx) and vitamin C was measured in 50 normal controls, 40 diabetics without complications, 22 diabetics with proliferative and 20 with ...

background: the aim of this study was to evaluate the possible protective effect of sodium selenite on serum, liver, and kidney antioxidant enzymes activities in alloxan-induced type 1 diabetic rats. methods: forty sprague-dawley male rats were randomly divided into four groups; group one as control, group two as sham-treated with sodium selenite by 1 mg/kg intraperitoneal (i.p.) injections dai...

Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in leaf crude extracts of different cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Liriope spicata L. were detected on 10% SDS-PAGE gels. All cultivars contained different Cu/Zn SOD isozymes with molecular masses between 30 and 50 kDa, which were identified by inhibitor tests. They also contained different GPx...

AIM To study the secretory activity and androgen regulation of glutathione peroxidase (GPx) in epithelial cell cultures from human epididymis. METHODS Tissue was obtained from patients undergoing therapeutic orchidectomy for prostatic cancer. Epithelial cell cultures were obtained from the caput, corpus and cauda epididymides. Enzymatic activity was measured in conditioned media by colorimetr...

Down syndrome or trisomy 21 is the single most common genetic cause of mental retardation. It has been postulated that there is oxidative stress in Down syndrome due to over expression of superoxide dismutase 1 (SOD -1) , an antioxidant enzyme which is coded on chromosome 21(21q22.1). The present study included 31 clinically diagnosed children with Down syndrome. The diagnosis of trisomy 21 was...

Superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS. In order to imitate the synergism of these enzymes, we designed and synthesized a novel 32-mer peptide (32P) on the basis of the prev...

Glutathione peroxidase (GPx) is an antioxidant protein containing selenium. Owing to the limitations of native GPx, considerable efforts have been made to develop GPx mimics. Here, a short 5-mer peptides (5P) was synthesized and characterized using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Enzyme coupled assays were used to evaluate GPx activity. The cell via...

The objective of the present study was to investigate effects different doses dietary Eucommia ulmoides leaf extract (ELE) on juvenile red claw crayfish (Cherax quadricarinatus). A total number 720 (initial body weight 0.24 ± 0.01 g) were randomly assigned six groups and fed diets containing 0 (Diet 1), 0.5 2), 1 3), 2 4), 4 5) 10 6) g dry (dw) ELE kg (dw)?1 for eight weeks challenged with micr...

Background Oxidant injury to the pulmonary endothelium contributes to acute lung injury. We have shown that activation of PKGI by cGMP increases protein levels of the antioxidant enzymes catalase and glutathione peroxidase-1 (GPx-1) and ameliorates oxidant injury in mouse lung endothelium [1]. Catalase and GPx-1 mRNA was not increased. The pathway downstream of PKGI that leads to increases in c...