I have read with interest the comment letter by Kruszelnicka et al. (1) to our article recently published in Diabetes (2). Their letter contains useful observations and raises interesting questions. One is about the dissociation of the metabolic sensitivity to insulin from endothelial nitric oxide (NO) release and its vascular protective effects, and the other is on the specificity of the assay...

INTRODUCTION Integrity of the great saphenous vein (GSV) endothelium is the most important key element for long-term patency rate of grafts in coronary artery bypass graft (CABG). Storage solutions play an important role in maintaining viability of vein endothelium. Diminished nitric oxide (NO) because of endothelial dysfunction may facilitate vascular inflammation and formation of atherosclero...

Abstract Several influenza pandemics have impacted our life, each with variable prevalence and severity. In a search for natural antivirals, further phytochemical investigation of Gardenia latifolia Aiton, Rubiaceae, was conducted. As result, five structurally diverse glycosides were isolated, offering valuable chemotaxonomic data. Using the crystal violet technique, three isolates, canthoside ...

Objective: To investigate the anti-inflammatory effects of total flavonoids from Saussurea involucrata on lipopolysaccharides (LPS)-stimulated murine RAW264.7 macrophages and explore its underlying mechanism action. Methods: Total were extracted using chromatographic column method. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The producti...

Background: Emphasis on substitutional medications with the elevated bacterial resistance to current antibiotics is pivotal. We evaluated antibacterial effect of AK2 by Minimum Bactericidal Concentration (MBC) and inhibitory (MIC) its impact macrophage responses in 17 strains pathogenic bacteria. The gene expression macrophage’s cytokines was evaluated. Accordingly, bioinformatic assessment pre...

Sohji Nagase, MD, PhD, Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Ten-nodai, Tsukuba, Ibaraki 305 (Japan), Tel/Fax +81 (298) 53-3202, E-Mail [email protected] Dear Sir, We read with great interest the report by Dr. Rivas-Cabañero et al. [1] published as a letter to the editor in Nephron last year. The authors found that nitrite and nitra...

hydroalcoholic extract (70% ethanol extract) of tubers of momordica tuberosa cogn. (cucurbitaceae) was subjected to preliminary phytochemical screening by qualitative tests. nitric oxide scavenging activity was performed by griess reagent method. and nephroprotective activity was assessed in gentamicin, cisplatin and paracetamol induced renal damage in wistar rats (150-200 g) by standard method...

Oxidation of nitric oxide in aqueous solution to nitrite but not nitrate: comparison with enzymatically formed nitric oxide from L-arginine. A spectrophotometric assay for nitrate using NADPH oxidation by Aspergillus nitrate reductase. Measurement of nitrite and nitrate in biological fluids by gas chromatography mass spectrometry and by the Griess assay: problems with the Griess assay—solutions...

In last decades, immunotherapies became more and significant as a cancer treatment method. Many in vivo vitro studies have been conducted the literature order to determine appropriate diagnostic methods for cancer. During development of therapeutic vaccines, determination vaccine concentration which is beginning step studies, critical healthy progression studies. Breast occurs by uncontrolled p...


 Purpose: To investigate the anti-inflammatory effects and antioxidant activity of Oroxylum indicum (L.) Kurz fruit extract in lipopolysaccharide (LPS)-stimulated BV2 microglia.
 Methods: cells were treated with LPS for 24 h presence or absence O. extract. Then, nitric oxide (NO), reactive oxygen species (ROS) interleukin 6 (IL-16) levels measured using Griess reagent assay, CM-H2DCF...