The effect of heat treatment on polyphenoloxidases and peroxidase activities in three tissues (cambium, central cylinder internal bark) the varietie cassava roots (Manihot esculenta CRANTZ) Bonoua 2 were studied over a range 30 to 70 °C, using pyrogallol, 4-methylcatechol, dopamine pyrocatechol as substrates for polyphenol oxydase guaiacol substrate peroxidase. Optimal conditions enzym...

The role of coated vesicles during the absorption of horseradish peroxidase was investigated in the epithelium of the rat vas deferens by electron microscopy and cytochemistry. Peroxidase was introduced into the vas lumen in vivo. Tissue was excised at selected intervals, fixed in formaldehyde-glutaraldehyde, sectioned without freezing, incubated in Karnovsky's medium, postfixed in OsO(4), and ...

Sensitivity of common onion (Allium cepa L.) and faba bean (Vicia faba L.) to cadmium (Cd) stress was investigated using genotoxicity endpoints. Simultaneously, the antioxidative stress enzymes (guaiacol peroxidise and catalase) and lipid peroxidation [malonaldehyde (MDA) content] were investigated in the plants exposed to Cd in solution. The endpoints screened for genotoxicity included chromos...

The activities of polyphenol oxidase (PPO) and peroxidase (POD) in hot pepper (Capsicum annuum L.) pericarp were evaluated using spectrophotometric method. The enzymes were extracted from the pepper pericarp 5 g with 20 mL of 0.1 M phosphate buffer solution pH 7. PPO and POD activities were determined using catechol and guaiacol as a substrate, respectively. The effects of the amounts of enzyme...

AIMS To investigate the occurrence and distribution of thermo-acidophilic bacteria (TAB) associated with various commercial fruit crop soils in Japan and to assess their ability to produce the odorous phenolic compound, guaiacol. METHODS AND RESULTS Phylogenetic analysis based on the 5' end of the 16S rRNA gene (approximately 500 bp), was performed on 62 TAB isolated from the soil of several ...

Wheat is a widely cultivated cereal throughout the world and stress caused by low temperatures significantly affects all stages of wheat development. Seed priming an effective method to produce stress-resistant plants. This work was carried out determine whether different methods (hormo-, halo-, osmo-, hydropriming) can increase resistance low-temperature conditions (10 °C). The effect on growt...

The present study aimed to investigate the effects of zinc exposure (0, 1, 5, 15, 30 mg/L zinc) on biochemical and physiological parameters Peganum harmala seedlings. Two populations (metallicolous non-metallicolous) were compared in Zn tolerance, accumulation, photosynthetic pigments, enzymatic antioxidant activities. Plants treated with at concentrations 0, for 14 days. results showed that in...

This work was focused on the role of silicon (Si) in amelioration of manganese (Mn) toxicity caused by elevated production of hydroxyl radicals (·OH) in the leaf apoplast of cucumber (Cucumis sativus L.). The plants were grown in nutrient solutions with adequate (0.5 μM) or excessive (100 μM) Mn concentrations with or without Si being supplied. The symptoms of Mn toxicity were absent in the lea...

Fruit from three genotypes (‘B-76’, ‘B-59’ and ‘SHF-3A’) of deerberry [Vaccinium stamineum L.] were evaluated for fruit quality, total anthocyanin and phenolic contents, antioxidants, antioxidant capacity, and antioxidant enzyme activity. The fruit soluble solids, titratable acids, total anthocyanins, and total phenolic contents varied with genotypes. Cyanidin 3-galactoside and cyanidin 3-arabi...