Haemophilus aegyptius and Haemophilus influenzae biotype III are morphologically and biochemically similar; however, their outer membrane protein (Sarkosyl insoluble) profiles are distinct. Of 18 strains of H. aegyptius examined, 15 had a type 1 protein profile, and 3 had a type 2 profile, whereas the 5 strains of H. influenzae biotype III examined had three other protein profile types. All Hae...

Bacteria of the family Pasteurellaceae are well known to exist in close relationship with mammalian hosts, mostly as constituents of commensal bacterial flora. However, at the same time, some species also have the ability to cause serious diseases. For example, Mannheimia haemolytica and Haemophilus somnus cause pneumonia in cattle (15); Pasteurella multocida is a common animal pathogen but occ...

Conjugation: Although H. influenzae genes may be transferred by plasmid-mediated conjugation, this process is not discussed further below as it has received little attention and is rarely used for strain construction. Conjugative plasmids are common in H. influenzae as in other bacteria [1, 2, 3]. The F plasmid of Escherichia coli can conjugate into H. influenzae cells [4] (and into many other ...

Haemophilus influenzae vaccine Hershel Jick, et al 107 Obstetric cholestasis Judith B Weaver 107 General practice-psychiatry liaison by audioconference John D Sheehan, et al 108 Emergency contraception Sue Ziebland and Pilar Garcia 108 Managing violence in the practice Brian A Kidd and Cameron Stark 109 Alzheimers Disease Society H WL Oliver 109 Communication in the year 2000 Brian O'Mahony 109...

Ampicillin resistance was first reported among clinical isolates of Haemophilus influenzae in 1972. Reports of chloramphenicol resistance followed shortly thereafter. The principal mechanism of resistance to these two antibiotics is enzymatic. Although other mechanisms have been described, they are found in comparatively few strains. The genetic information for the inactivating enzymes is plasm...

gations: chest x-ray normal; Hb 55%; W.B.C. 7,100 with normal diffierential; iron deficiency on the blood film; E.S.R. 138 mm. in one hour; Paul-Bunnell test negative. Blood culture revealed Haemophilus influenzae type B sensitive to all the usual antibiotics except sulphonamide. This child was treated with ampicillin and made a good recovery. My point in recording this case history is to empha...

A culture medium for the selective isolation of Haemophilus species is described. Bacitracin and nutritional supplements were incorporated in a rich basal agar medium to which rabbit blood was added to distinguish hemolytic species. Colony counts of seven typed strains of H. influenzae on this medium were within practical limits of counts on other media tested for clinical use. The bacitracin m...

Plackett-Burman experimental design was used to screen important medium components influencing the production of polyribosyl ribitol phosphate by Haemophilus influenzae type-B under submerged fermentation. Eleven media parameters such as Glutamic acid, Yeast extract, cystein, dextrose, NAD, heamin, NaCl, NH4Cl, Na2HPO4, KCl, and MgSO4.7H2O were screened in twelve experimental runs as per the de...

conclusions we found ccd and rsm very effective in optimizing hib culture conditions, and hib biomass production was greatly influenced by ph and incubation temperature. therefore, optimization of the growth factors to maximize hib production can lead to 1) an increase in bacterial biomass and prp productions, 2) lower vaccine prices, 3) vaccination of more susceptible populations, and 4) lower...

We studied genetic diversity in Streptococcus pneumoniae and Haemophilus influenzae in throat culture isolates from 38 children attending two day-care centers in Michigan. Culture specimens were collected weekly; 184 S. pneumoniae and 418 H. influenzae were isolated from the cultures. Pulsed-field gel electrophoresis identified 29 patterns among the S. pneumoniae isolates and 87 among the H. in...