We detected an antibody to HCV envelope protein (E1) in sera of patients with HCV-related chronic liver diseases (20 patients with chronic hepatitis and 5 patients with liver cirrhosis) by Western blotting using the fusion protein of E1 envelope protein and beta-galactosidase as an antigen. The antibody to HCV E1 (anti-HCV E1) was detected in 8 (42%) of 19 patients positive for HCV-RNA (16 were...

For simplicity we adopt the following convention: I, X denote sets, M denotes a many sorted set indexed by I, R1 denotes a binary relation on X, and E1, E2, E3 denote equivalence relations of X. We now state the proposition (1) (E1 ⊔ E2) ⊔ E3 = E1 ⊔ (E2 ⊔ E3). Let X be a set and let R be a binary relation on X. The functor EqCl(R) yielding an equivalence relation of X is defined as follows: (De...

Prophylactic hepatitis C virus (HCV) vaccine trials with human volunteers are pending. There is an important need for immunological end points which correlate with vaccine efficacy and which do not involve invasive procedures, such as liver biopsies. By using a multicomponent DNA priming-protein boosting vaccine strategy, naïve chimpanzees were immunized against HCV structural proteins (core, E...

ion and function application employ the notion of substitution. Recall the inference rule and its representation in LF: e1 ↪→ lam x. e1 e2 ↪→ v2 [v2/x]e1 ↪→ v ev app e1 e2 ↪→ v ev app : ΠE1:exp. ΠE2:exp. ΠE′ 1:exp→ exp. ΠV2:exp. ΠV :exp. eval E1 (lam E′ 1) → eval E2 V2 → eval (E′ 1 V2) V → eval (app E1 E2) V. As before, we transcribe this (and the trivial rule for evaluating λ-expressions) into...

We are developing an orally available small-molecule, allosteric TSH receptor (TSHR) agonist for follow-up diagnostics of patients with thyroid cancer. The agonist C2 (NCGC00161870) that we have studied so far is a racemic mixture containing equal amounts of two enantiomers, E1 and E2. As enantiomers of many drugs exhibit different pharmacologic properties, we assessed the properties of E1 and ...

background: hepatitis c virus (hcv) is a main public health problem causing chronic liver infection and subsequently liver cirrhosis and lethal hepatocellular carcinoma (hcc). vaccination based on hcv capsid proteins has attracted a special interest for prevention of viral infections. the core protein is a basic and evolutionary most conserved protein, which regulates the cellular processes rel...

UNLABELLED The measure of changes in synaptic dopamine (DA) concentration in response to the psychostimulant drug methylphenidate (MP) has been used as an indicator of responsiveness of the DA system. The purpose of this study was to assess the reproducibility of these measures. METHODS Seven healthy subjects were scanned with PET and [11C]raclopride twice in the same day: 7 min after placebo...

The mechanism of DNA replication is conserved among papillomaviruses. The virus-encoded E1 and E2 proteins collaborate to target the origin and recruit host DNA replication proteins. Expression vectors of E1 and E2 proteins support homologous and heterologous papillomaviral origin replication in transiently transfected cells. Viral proteins from different genotypes can also collaborate, albeit ...

BACKGROUND Only about 50% of patients chronically infected with HCV genotype 1 (HCV-1) respond to treatment with pegylated interferon-alfa and ribavirin (dual therapy), and protease inhibitors have to be administered together with these drugs increasing costs and side-effects. We aimed to develop a predictive model of treatment response based on a combination of baseline clinical and viral para...