We report the isolation and characterization of a cloned DNA of D. melanogaster, Dm4L, that is derived from the major heat shock puff site at 63B. This segment contains two closely linked genes that are each present once per Drosophila haploid genome. One of these, the hsp 83 gene, encodes an abundant heat shock mRNA that, unlike other major heat shock mRNAs, is also abundant in uninduced (23 d...

Protein-damaging stresses, including heat shock, cold, altered pH and oxygen deprivation induce the expression of a subgroup of molecular chaperones, called heat shock proteins (Hsp), which consist of several protein families designated by their molecular weight, such as the Hsp90, Hsp70, Hsp60, and the small Hsp (sHsp) families. Small heat shock proteins (sHsp) with a molecular mass of 15–30 k...

The expression of the Mr 70,000 heat shock protein (HSP-70) in heat-resistant variants or heat-shocked cells has been correlated with development of thermal resistance. In these studies polymerase chain reaction (PCR) was used to detect low levels of HSP-70 mRNA present in control, unheated cells to investigate the possibility of predicting the intrinsic heat response in various leukemic cells....

A study was undertaken to investigate the effect of melatonin supplementation on heat shock proteins (HSPs) (HSP 60, HSP70, HSP 90 and 110) expression profile in buffalo calves under summer stress. Twelve healthy Murrah male 6 month 1 year age group were taken for study. Buffalo divided into control (CG) treatment (TG) group. In TG, Melatonin (18mg/50 kg BW) injected subcutaneously, two times a...

background: wet cupping therapy is a complementary therapy in pain management. the mechanism of this therapy, however, needs further elucidation. cells injured by wet cupping therapy seem to stimulate the expression of heat shock protein 70 (hsp70). its benefit in pain reduction could be mediated by the expression of ß-endorphin. this study aimed at determining the correlation between hsp70 and...

luteolin, as a natural polyphenolic compound, has neuroprotective effect and exerts its function by attenuation of apoptosis and oxidative stress factors. emerging evidences indicate that oxidative stress leads to neurodegeneration but is not the initial event and endoplasmic reticulum stress (er) is often considered to be the stimulus event which is caused by accumulating of misfolded proteins...

Effective vaccination using in vitro peptide-loaded heat-shock proteins (HSP), tumor-derived HSP and HSP fusion proteins has been shown in viral, parasite and tumor model systems. We demonstrate protective DNA vaccination using gp96-peptide fusion proteins against the intracellular bacterial pathogen Listeria monocytogenes in a mouse model. In contrast to previous studies using pathogen-derived...

Replication-competent viruses are currently being evaluated for their cancer cell-killing properties. These vectors are designed to induce tumor regression after selective viral propagation within the tumor. However, replication-competent viruses have not resulted heretofore in complete tumor eradication in the clinical setting. Recently, heat shock has been reported to partially alleviate repl...

(1) Treatment of resident peritoneal macrophages for 8 h with macrophage colony-stimulating factor (M-CSF) increased release of superoxide anion (O2-) stimulated by phorbol 12-myristate 13-acetate. Gel electrophoresis of pulse-labelled proteins with L-[35S]methionine showed that a number of proteins were induced during activation by M-CSF. Immunoblot analysis with antibody against heat shock pr...