Background and Aims: Recent studies have shown the anticancer properties of metformin, which is widely used in diabetes mellitus. The possible mechanisms effects metformin not been fully elucidated. We aimed to investigate cytotoxic, genotoxic, apoptotic HepG2 HeLa cells. Methods: cytotoxicity, genotoxicity, apoptotoic were determined by MTT method, Comet assay, FACS respectively. Results: Met...

In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping enhances...

Human adenovirus type 5 enhances the thymidine kinase activity of KB cells but does not induce the enzyme in kinase-deficient HeLa (BU25) cells. Vaccinia induces thymidine kinase activity in both KB and HeLa (BU25) cells. Human adenovirus types 2, 4, 7, and 12 also fail to induce the enzyme in HeLa (BU25) cells. Vaccinia replicates equally well in the presence or absence of HATG (hypoxanthine-a...

Reactive oxygen species (ROS) act as a mediator of ionizing radiation-induced cellular damage. Previous studies have indicated that MnSOD (SOD2) plays a critical role in protection against ionizing radiation in mammalian cells. In this study, we constructed two types of stable HeLa cell lines overexpressing SOD2, HeLa S3/SOD2 and T-REx HeLa/SOD2, to elucidate the mechanisms underlying the prote...

Suberoylanilide hydroxamic acid (SAHA) is a histone deacetylase (HDAC) inhibitor which has anticancer effects. We evaluated the growth inhibitory effects of SAHA on HeLa cervical cancer cells in relation to reactive oxygen species (ROS) levels. SAHA inhibited the growth of HeLa cells with an IC(50) of approximately 10 µM at 24 h, and induced apoptosis which was accompanied by the cleavage of PA...

Abstract: Aim/Background: Metastasis is the main reason of death in cancer with a key challenge to target it precisely by available treatments. The current study was designed assess anti-metastatic potentials synthesized chalcones, Ch-1 and Ch-2. Materials Methods: In vitro included cytotoxicity, scratch wound assay cell cycle analysis vivo performed C57BL/6 mice B16-F10 induced metastatic lung...