We have developed methodology that allows comparison of the mean corpuscular hemoglobin (MCH) of fetal hemoglobin (HbF)-containing red cells (F cells) with the MCH of non-F cells from the same individual. To do this, suspensions of peripheral blood erythrocytes and their internal contents are fixed with an imidodiester, dimethyl-3,3'-dithiobispropionimidate dihydrochloride (DTBP). Thereafter fi...

The hemoglobins present in murine fetal hepatic erythroblasts on days 12-15 of gestation were studied by biochemical and immunocytologic techniques. In addition, fetal hepatic hemopoietic progenitor cells obtained from normal and mutant f/f mouse fetuses on days 11-13 of gestation were cultured in vitro with added erythropoietin and adult spleen cell conditioned medium to form large erythroid c...

We compare and discuss three electrophoretic methods for identifying hemoglobins S, A, C, F, and D or G. Electrophoresis on citrate agar gel was more sensitive than electrophoresis on cellulose acetate for detecting hemoglobins S and F, a fundamental consideration in designing cord-blood screening programs for detecting hemoglobin S carriers. Electrophoresis on starch gel is evidently an accept...

H UMAN and several other mammalian species sequentially express different globin genes during ontogeny, or during periods of “stress,” such as hypoxemia or anemia. Hemoglobin ontogeny in humans includes three distinct phases. The “embryonic” phase predominates from the 5th to the 16th week of gestation. In this period, embryonic hemoglobin chains are and . Throughout the remainder of gestation ...

Reactions of Oxygen with Hemoglobin, Cytochrome C Oxidase and Other Hemeproteins. By W. S. Caughey, C. H. Barlow, J. C. Maxwell, J. A. Volpe, and W. J. Wallace 1 Measurement of Structural and Free Energy Changes in Hemoglobin by Hydrogen Exchange Methods. By S. W. Engländer 10 Leghemoglobin: The Role of Hemoglobin in the Nitrogen-Fixing Legume Root Nodule. By Jonathan B. Wittenberg, C. A. Apple...